Glycerides, C16-22

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

From Jun. 1988 to Jul. 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to recognized methodology but not under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Robra K.H. (1976). Wasser/Abwasser 117:80-86

Deviations:

not specified

Principles of method if other than guideline:

Bacteriotoxic substances inhibit the bacteria's enzymatic activity, leading to decrease oxygen consumption. The Robra-test measures oxygen consumption of Pseudomonas putida cultures exposed to various concentrations of a test substance and the EC10 or EC50 compared to a negative controlare determined.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Glycerides, C16-18 and C18 unsatd.

IUPAC Name:

Glycerides, C16-18 and C18 unsatd.

Constituent 2

Reference substance name:

67701-30-8

Cas Number:

67701-30-8

IUPAC Name:

67701-30-8

Constituent 3

Reference substance name:

Glycerides, C16-18 and C18-unsatd.

EC Number:

266-948-4

EC Name:

Glycerides, C16-18 and C18-unsatd.

IUPAC Name:

266-948-4

Details on test material:

- Name of test material (as cited in study report): Alkali-refined linseed oil (CAS N° 8001-26-1, EC N° 232-278-6). Under the SDA nomenclature, the name of this substance is 'Glycerides, C16-18 and C18 unsatd.'

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

No data

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Laurylmyritylalcoholethoxylate 7.5 E.O

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas DSM putida 50026
- Method of cultivation: in standard nutrient solution
- Preparation of inoculum for exposure: Pre-inoculum cultivated at 1°C for ca. 16 h on a rotary shaker. 10 ml pre-cuture taken and dissolved in 200 ml nutrient solution, shaken at 26°C for ca. 16 h prior to test start.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Remarks on exposure duration:

none

Post exposure observation period:

No data

Test conditions

Hardness:

Not applicable

Test temperature:

Not applicable

pH:

Not applicable

Dissolved oxygen:

Not applicable

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 0, 40,000, 50,000, 60,000, 70,000, 80,000, 90,000 and 100,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 300 ml Erlenmeyer
- Aeration: with pressurized air

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

67 000 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

None

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

None

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of this test, the EC10 of alkali-refined linseed oil to Pseudomonas putida was estimated to be 67,000 mg/L.

Executive summary:

A study was conducted to evaluate the toxicity of linseed oil to Pseudomonas putida according to the method described in Robra K.H. (1976). GFW Wasser/Abwasser 117:80 -86.

The microorganisms were exposed to concentrations of the test substance ranging from 40,000 to 100,000 mg/L and oxygen consumption was measured.

Under the conditions of this test, the EC10 of the test substance to Pseudomonas putida was estimated to be 67,000 mg/L.