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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD Guideline no. 202 and in compliance with GLP
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
NA
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
not specified
Details on test material:
Common name 4,4’-oxybis(benzenesulfonyl hydrazide)
other: purity Sigma-Aldrich Co., Purity: 95.3%
other: Lot no. 06430CC
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
NA

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: OECD M4 Medium
Hardness: 243 mg/L as CaCO3
Alkalinity: 33.1 mg/L as CaCO3

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test Organism
Age: Juveniles within 24 hrs old
Supplier: GSF Institute of Ecological Chemistry, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no

Test conditions

Hardness:
243 mg/L as CaCO3
Test temperature:
20.1± 0.2 °C
pH:
7.43 ~ 7.92
Dissolved oxygen:
7.3 ~ 8.7 mg/L (80 ~ 96 % of the air saturation value)
Salinity:
NA
Nominal and measured concentrations:
Nominel concentrations:
Control; 0.3 mg/L; 0.6 mg/L; 1 mg/L; 3 mg/L; 5 mg/L; 10 mg/L; 20 mg/L; 40 mg/L
Meassured concentrations:
0.05, 0.07, 0.10, 0.20, 0.38, 0.52, 0.81, and 1.37 mg/L (3.43 % ~ 16.7 % of nominal concentration)
Details on test conditions:
Dilution water source: OECD M4 medium (Hardness: 243 mg/L as CaCO3, Alkalinity: 33.1 mg/L as CaCO3)
Water chemistry: DO = 7.3 ~ 8.7 mg/L (80 ~ 96 % of the air saturation value), pH = 7.43 ~ 7.92
Temperature: 20.1± 0.2 °C
Light: 750 ~ 860 Lux, Light periodicity: 16/8 (light/dark)

Replicates: 3 replicates
Loading: 10 daphnids/concentration
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
23 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
15 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Result: 48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 15 mg/L (13 ~ 18 mg/L). The NOEC was estimated to be 5 mg/L at 48 hrs.

Control: No immobilisation and adverse effects were observed in the control.
At 24 hrs, 14 and 26 daphnids were immobilized with 20 and 40 mg/L of OBSH, respectively. At 48 hrs, 6, 20, and 30 daphnids were immobilized with 10, 20, and 40 mg/L of OBSH. Any abnormal behaviors of the daphnids was notobserved in each treatment group during the testing.

Precipitation of the test substance and formation of film at the surface were observed in more than 1 mg/L but immobilisation was observed in more than 10 mg/L. Therefore immobilization of daphnia and film formation are not related to each other.
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
The EC50 value (α=0.05) were calculated by Probit Analysis method (EPA/600/4-85/013, 1985).

Any other information on results incl. tables

In the stability test, the concentration of OBSH was decreased by 50% ofthe initial level (1 mg/L) after 4 hrs in daphnia culture media The measured concentration of test substance was very low because of its low water solubility and high degradation of dissolved substance in aqueous solution.At 48 hrs (the end of the test), the mean measured concentrations of OBSH were 0.05, 0.07, 0.10, 0.20, 0.38, 0.52, 0.81, and 1.37 mg/L (3.43 %~16.7 % of nominal concentration).Although several peaks for degraded or transformed products were observed, identification and measurement of them were not performed. Therefore, all test results were expressed as nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 15 mg/L (13 ~ 18 mg/L). The NOEC was estimated to be 5 mg/L at 48 hrs.
Executive summary:

The Toxicity of OBSH towards Daphnia magna was tested applying the OECD Guideline no. 202. The study was conducted accoring to GLP. Test concentrations were measured at the beginning (0 hr) and the end (48 hrs) of the studyusing HPLC with C18 column and DAD G1315B detector. Based on the endpoint mobility a 48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 15 mg/L (13 ~ 18 mg/L). The NOEC was estimated to be 5 mg/L at 48 hrs.