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EC number: 205-594-7 | CAS number: 143-24-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-05-21 to 2012-06-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline Study; EU method; GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis(2-(2-methoxyethoxy)ethyl) ether
- EC Number:
- 205-594-7
- EC Name:
- Bis(2-(2-methoxyethoxy)ethyl) ether
- Cas Number:
- 143-24-8
- Molecular formula:
- C10H22O5
- IUPAC Name:
- 2,5,8,11,14-pentaoxapentadecane
- Reference substance name:
- Tetraglyme
- IUPAC Name:
- Tetraglyme
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test media (containing algae) of all test concentrations and from the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control (containing algae) were taken at the end of the test (after the 72 hours test period).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Dosage of Test Item:
The test medium of the highest test concentration of nominal 10000 mg test item/L was prepared by dissolving 7001.5 mg test item into 700.15 mL test water by intense stirring for 15 minutes. Adequate volumes of this test medium were diluted with test water to prepare the test media of the other desired test concentrations.
The test media were prepared just before introduction of the algae (= start of the test).
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- SSpecies: Pseudokirchneriella subcapitata, Strain No. 61.81 SAG
Origin: The algae were supplied by the "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen", 37073 Göttingen, Germany.
Breeding Conditions: The algae were cultivated in the laboratories of IBACON under standardised conditions according to the test guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Calculated water hardness of the test water: 0.24 mmol/L (= 24 mg/L) as CaCO3
- Test temperature:
- 22 to 23°C
- pH:
- 8.1 at test start and
8.0 to 8.7 at test end - Nominal and measured concentrations:
- Test Concentrations:
10000, 5000, 2500, 1250 and 625 mg test item/L and a control. - Details on test conditions:
- Water Temperature: The temperature was measured daily in an Erlenmeyer flask filled with water and incubated under the same conditions as the test flasks.
Water temperature was:
22 to 23 °C
pH-Values: The pH was measured in all test item concentrations and the control at the start and the end of the test.
pH was:
8.1 at test start and
8.0 to 8.7 at test end
Light Regime: Continuous illumination
Light Intensity: The light intensity was measured once during the test at 6 positions distributed over the experimental area at the surface of the test media.
Mean light intensity:
6103 lux (range: 5680 to 6680 lux) - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 996 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL:8211-10048
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2 871 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 2283 - 3378
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 625 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 814 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL:2495 - 3177
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 769 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL:568- 959
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 625 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- The 72-hour ErC50 value was calculated to be 8996 mg test item/L and the 72-hour EbC50 was calculated to be 2814 mg test item/L. The 72-hour NOErC and the 72-hour NOEbC were determined to be lower than 625 mg test item/L and the associated 72-hour LOEC for both parameters is 625 mg test item/L at most.
- Reported statistics and error estimates:
- Based on the calculated cell densities, the 72-hour ErC50 and the 72-hour EyC50, the corresponding EC20 and EC10 values and their 95 %-confidence limits were calculated by Probit analysis.
For the determination of the 72-hour LOEC and the 72-hour NOEC, the calculated growth rates and yields at each test concentration were tested for significant differences compared to the control values by the Welch t-test (growth rate) and the Williams t-test (yield), respectively.
The software used to perform the statistical analysis was ToxRat Professional, Version 2.10.05, ToxRat Solutions GmbH.
Any other information on results incl. tables
Biological Results
Yield y and Percentage Inhibition of y during the Test Period
Nominal concentration | Yields y [104cells/mL] and % inhibition of y | ||||||||
24 hours | 48 hours | 72 hours | |||||||
[mg test item/L] | y | % | y | % | y | % | |||
Control | 3.871 | - | 29.496 | - | 109.217 | - | |||
625 | 5.086 | -31.4 | 28.471 | 3.5 | 98.246 | 10.0 | * | ||
1250 | 3.795 | 2.0 | 23.232 | 21.2 | * | 82.074 | 24.9 | * | |
2500 | 2.352 | 39.2 | * | 17.765 | 39.8 | * | 66.130 | 39.5 | * |
5000 | 1.289 | 66.7 | * | 7.819 | 73.5 | * | 31.887 | 70.8 | * |
10000 | 0.986 | 74.5 | * | 2.504 | 91.5 | * | 5.086 | 95.3 | * |
negative values in ‘% inhibition’ indicate an increase in growth relative to that of the control
* mean value significantly different from the control(tested with Williams t-test,a = 0.05, one-sided)
Growth Rates µ and Percentage Inhibition of µ during the Test Period
Nominal concentration | Growth rates µ [1/day] and % inhibition of µ | ||||||||
0 - 24 hours | 0 - 48 hours | 0 - 72 hours | |||||||
[mg test item/L] | µ | % | µ | % | µ | % | |||
Control | 2.161 | - | 2.046 | - | 1.796 | - | |||
625 | 2.412 | -11.6 | 2.030 | 0.8 | 1.762 | 1.9 | * | ||
1250 | 2.148 | 0.6 | 1.930 | 5.7 | * | 1.701 | 5.3 | * | |
2500 | 1.739 | 19.5 | * | 1.799 | 12.1 | * | 1.630 | 9.2 | * |
5000 | 1.270 | 41.2 | * | 1.406 | 31.3 | * | 1.390 | 22.6 | * |
10000 | 1.079 | 50.1 | * | 0.894 | 56.3 | * | 0.789 | 56.1 | * |
negative values in ‘% inhibition’ indicate an increase in growth relative to that of the control
* mean value significantly different from the control(tested with Welch t-test,a = 0.05, one-sided)
Analytical Results
Determination of the Test Item: | Based on the results of GC measurements the concentration of the test item was determined using a calibration curve. | ||||||||||||||||||||||||||||||||||||||||||||
Calibration: | 0.5 to 10 mg test item/L | ||||||||||||||||||||||||||||||||||||||||||||
Linearity of Response: | Correlation of peak area of different standard solutions with their corresponding concentrations, using a linear regression. | ||||||||||||||||||||||||||||||||||||||||||||
Regression Coefficient: | At least 0.9986 | ||||||||||||||||||||||||||||||||||||||||||||
Calibration Curve: | y = 38846 * x - 2788 | ||||||||||||||||||||||||||||||||||||||||||||
Limit of Detection: | 0.048 mg test item/L | ||||||||||||||||||||||||||||||||||||||||||||
Limit of Quantification: | 250 mg test item/L mean recovery rate: 102 % (n = 5; RSD 2 %) | ||||||||||||||||||||||||||||||||||||||||||||
Mean Recovery in the Fortified Samples: |
| ||||||||||||||||||||||||||||||||||||||||||||
Mean Recovery in the Test Samples: |
After 72 h: 107 % (n = 10, RSD 5 %)
| ||||||||||||||||||||||||||||||||||||||||||||
| Table3. Summary of Analytical Results
1mean value of all measured samples per treatment group | ||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||
The limit of detection and the limit of the quantification of the analytical method were 0.048 and 250 mg test item/L, respectively. At the start of the test 108 % of the nominal test concentrations were found (average of all test concentrations). After 72 hours test duration, 107 % of the nominal value was determined (average of all test concentrations). During the test the algae were exposed to a mean of 108 % of nominal. Therefore, all reported results refer to nominal concentrations. |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The quantification of the test item was performed using gas chromatography (GC-MS-method). The limit of detection and the limit of the quantification of the analytical method were 0.048 and 250 mg test item/L, respectively.
At the start of the test 108 % of the nominal test concentrations were found (average of all test concentrations). After 72 hours test duration, 107 % of the nominal value was determined (average of all test concentrations). During the test the algae were exposed to a mean of 108 % of nominal. Therefore, all reported results refer to nominal concentrations.
The influence of Tetraethyleneglycoldimethylether on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour ErC50 value was calculated to be 8996 mg test item/L and the 72-hour EbC50 was calculated to be 2814 mg test item/L. The 72-hour NOErC and the 72-hour NOEbC were determined to be lower than 625 mg test item/L and the associated 72-hour LOEC for both parameters is 625 mg test item/L at most. - Executive summary:
The toxicity of the test item to Pseudokirchneriella subcapitata in an algal growth inhibition test was determined based on the procedures indicated by the following internationally accepted method:
- OECD Guideline No 201
- EU Method C.3
The influence of the test item on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. Exponentially growing cultures of this unicellular green algal species were exposed to varios concentrations of the test item under defined conditions.The inhibition growth in relation to control cultures was determined over a test period of 72 hours, and thus over several algal generations.
Test Concentrations: 10000, 5000, 2500, 1250 and 625 mg test item/L and a control.
T The influence of Tetraethyleneglycoldimethylether on the growth of the freshwater green algaePseudokirchneriella subcapitatawas assessed in a static dose-response test.The 72-hour ErC50value was calculated to be8996 mg test item/L and the 72-hour EbC50was calculated to be2814 mg test item/L. The 72-hour NOErC and the 72-hour NOEbC were determined to be lower than 625 mg test item/L and the associated 72-hour LOEC for both parameters is 625 mg test item/L at most.
This study is classified acceptable and satisfies the guideline requirements for growth inhibition algae studies.
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