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Registration Dossier
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EC number: 201-247-9 | CAS number: 80-07-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Additional ecotoxological information
Administrative data
- Endpoint:
- additional ecotoxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented study which meets generally accepted scientific standards
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Screening of some anti-androgenic endocrine disruptors using a recombinant cell-based in vitro bioassay
- Author:
- Roy P, Salminen H, Koskimies P, Simola J, Smeds A, Saukko P & Huhtaniemi IT
- Year:
- 2 004
- Bibliographic source:
- PMID: 15084347, J Steroid Biochem Mol Biol. 88(2):157-66
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The measurement was made employing a Cell-based androgen reporter assay using the Chinese hamster ovarian cell line (CHO K1) in the 96 -well format. The purpose is high throughput screening for endocrine disrupting (ED) substances in environmental and biological samples. To this end, CHO cells were cotransfected with plasmids encoding mouse mammary tumour virus-neomycin-luciferase and human androgen receptor (hAR), and a stable cell line was established. After selection with neomycin, a highly active clone was obtained which stably expressed both the hAR and the androgen-responsive luciferase reporter.
- GLP compliance:
- no
- Type of study / information:
- Endocrine effects
Test material
- Reference substance name:
- Bis(4-chlorophenyl) sulphone
- EC Number:
- 201-247-9
- EC Name:
- Bis(4-chlorophenyl) sulphone
- Cas Number:
- 80-07-9
- Molecular formula:
- C12H8Cl2O2S
- IUPAC Name:
- 1-chloro-4-(4-chlorobenzenesulfonyl)benzene
- Details on test material:
- - Name of test material (as cited in study report): 4,4'-Dichlorodiphenyl sulphone
Constituent 1
Results and discussion
Any other information on results incl. tables
Tested for androgenic or anti-androgenic effects, DCDPS gave in vitro a negative response.
None of the tested compounds appeared to have any androgenic activity in the cell line up to the concentration of 50 µmol/L, when tested alone.
Applicant's summary and conclusion
- Conclusions:
- Tested for anti-androgenic and androgenic effects, DCDPS gave in vitro a negative response at a concentration of 0.1 µmol/L = 0.287 ng/L.
- Executive summary:
The development and optimization of a cell-based androgen reporter assay using the Chinese hamster ovarian cell line (CHO K1) in the 96 -well format is described. The intent is a use as screening system for identifying endocrine disrupting substances (ED) in a high throughput manner in environmental and biological samples. To this end, CHO cells were cotransfected with plasmids encoding mouse mammary tumour virus-neomycin-luciferase and human androgen receptor (hAR), and a stable cell line was established. After selection with neomycin, a highly active clone was obtained which stably expressed both the hAR and the androgen-responsive luciferase reporter. Stimulation of the cells with androgens for 24 h resulted in about 15-fold stimulation of luciferase activity, with the minimum effective dose of testosterone being 0.1 nmol/L. Potent steroidal and non-steroidal anti-androgens, such as hydroxyflutamide and cyproterone acetate, significantly inhibited the androgen-induced transactivation. Non-androgenic steroids like estradiol, progesterone, dexamethasone and cortisol showed weak activity at high concentrations. RT-PCR and western blot confirmed proper transcription and translation as well as stable expression of the AR gene in the cells.
About 60 different substances (mostly pesticides or their metabolites, and common industrial chemicals including DCDPS) were screened with the cell line for their ability to stimulate luciferase activity (indicating androgenic activity) or inhibit that evoked by 0.1 nmol/L R1881 (indicating anti-androgenic activity), used as a positive androgenic control. About 10 highly potent anti-androgenic chemicals were identified. The most potent anti-androgenic compounds identified included bisphenol A, α-hexachlorocyclohexane, vinclozolin and 4,4-DDE (CAS 72-55-9), a structural analogue to DCDPS, which gave a negative response. These compounds had alone either no effect or were weak agonists (with cytotoxic effects at very high concentrations), but none showed any significant agonistic activity. None of the tested compounds appeared to have any androgenic activity in the cell line up to the concentration of 50 µmol/L, when tested alone.
In conclusion, it is demonstrated the bioassay based on this cell line provides a reliable test for detecting androgenic and anti-androgenic compounds. DCDPS was not active as androgen or anti-androgen endocrine disruptor.
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