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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1994

Materials and methods

Principles of method if other than guideline:
The mutagenic potency of the test substance in Tradescantia was examined.
GLP compliance:
no

Test material

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
Vehicle: 0.2 M citrate-phosphate buffer (pH = 5)
Test substance purchased from Sigma Chemical Co. (St. Louis, MO, USA)

Test organisms

Species:
other: Tradescantia clone 4430
Details on test organisms:
Hybrid of T. subacaulis X T. hirsutiflora cultivated at the Institute of Experimental Botany, Prague

Study design

Test type:
other: Mutagenicity assay
Study type:
laboratory study
Total exposure duration:
15 d
Post exposure observation period:
Mutations scored on days 9-14 post-exposure

Test conditions

Nominal and measured concentrations:
0 (citrate phosphate buffer only), 25, 50, 100, 125, 150 and 200 mM

Results and discussion

Effect concentrations
Species:
other: Tradescantia
Dose descriptor:
other: mutagenic potency
Effect conc.:
0.46 other: stamen hair mutants/µmole.

Any other information on results incl. tables

A significant increase in pink stamen hair mutations was seen only in the 125 and 150 mM treatment groups. The test substance was found to be toxic at concentrations above 150 mM, and repressed flowering. At 150 mM, the test substance induced a 3-fold increase in the mutant frequency over the spontaneous control.

Applicant's summary and conclusion

Conclusions:
Under the test conditions employed in this study, the test substance was found to be mutagenic in Tradescantia clone 4430 stamen hair cells when compared to the control. The mutagenic potency of the test substance in Tradescantia was 0.46 stamen hair mutants/µmole.
Executive summary:

The test substance was tested in a mutagenic assay in plants at concentrations of 0 (citrate phosphate buffer only), 25, 50, 100, 125, 150, and 200 mM. A significant increase in pink stamen hair mutations was seen only in the 125 and 150 mM treatment groups. The test substance was found to be toxic at concentrations above 150 mM, and repressed flowering. At 150 mM, the test substance induced a 3-fold increase in the mutant frequency over the spontaneous control. Under the test conditions employed in this study, the test substance was found to be mutagenic in Tradescantia clone 4430 stamen hair cells when compared to the control. The mutagenic potency of the test substance in Tradescantia was 0.46 stamen hair mutants/ µmole.