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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Single dose tested. No controls employed. Insufficient detail on method to adequately evaluate results.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1987

Materials and methods

Principles of method if other than guideline:
A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability study in freshly isolated mouse thymocytes were performed.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Test animals

Species:
other: Mouse embryo fibroblasts (BALB/c3T3) cells, BHK 21/C13 cells, and Rat thymocytes

Results and discussion

Details on results:
Neutral Red Uptake Test: NR-50 = 15.0 µg/mL

Cell Detachment from Culture Dish: CD-25 = 300 µg/mL

Growth Inhibition Assay: 2 µg/mL

Membrane Permeability Assay: FS-25 = 26 µg/mL, VR-25 >3200 µg/mL

Applicant's summary and conclusion

Executive summary:

A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability assay in freshly isolated mouse thymocytes were performed. The exposure times were 24 hours, 4 hours, 48 hours, and 20 minutes for the neutral red uptake test, cell detachment assay, growth inhibition assay, and membrane permeability assay, respectively.