Registration Dossier
Registration Dossier
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EC number: 203-404-7 | CAS number: 106-50-3
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Single dose tested. No controls employed. Insufficient detail on method to adequately evaluate results.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 987
Materials and methods
- Principles of method if other than guideline:
- A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability study in freshly isolated mouse thymocytes were performed.
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
Constituent 1
Test animals
- Species:
- other: Mouse embryo fibroblasts (BALB/c3T3) cells, BHK 21/C13 cells, and Rat thymocytes
Results and discussion
- Details on results:
- Neutral Red Uptake Test: NR-50 = 15.0 µg/mL
Cell Detachment from Culture Dish: CD-25 = 300 µg/mL
Growth Inhibition Assay: 2 µg/mL
Membrane Permeability Assay: FS-25 = 26 µg/mL, VR-25 >3200 µg/mL
Applicant's summary and conclusion
- Executive summary:
A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability assay in freshly isolated mouse thymocytes were performed. The exposure times were 24 hours, 4 hours, 48 hours, and 20 minutes for the neutral red uptake test, cell detachment assay, growth inhibition assay, and membrane permeability assay, respectively.
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