p-phenylenediamine

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Single dose tested. No controls employed. Insufficient detail on method to adequately evaluate results.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1987

Materials and methods

Principles of method if other than guideline:

A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability study in freshly isolated mouse thymocytes were performed.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Test animals

Species:

other: Mouse embryo fibroblasts (BALB/c3T3) cells, BHK 21/C13 cells, and Rat thymocytes

Results and discussion

Details on results:

Neutral Red Uptake Test: NR-50 = 15.0 µg/mL

Cell Detachment from Culture Dish: CD-25 = 300 µg/mL

Growth Inhibition Assay: 2 µg/mL

Membrane Permeability Assay: FS-25 = 26 µg/mL, VR-25 >3200 µg/mL

Applicant's summary and conclusion

Executive summary:

A neutral red uptake test in mouse embryo fibroblast cells, a cell detachment from culture dish in BHK 21/C13 cells, a growth inhibition assay in BHK 21/C13 cells, and a membrane permeability assay in freshly isolated mouse thymocytes were performed. The exposure times were 24 hours, 4 hours, 48 hours, and 20 minutes for the neutral red uptake test, cell detachment assay, growth inhibition assay, and membrane permeability assay, respectively.