Allyl 2,3-epoxypropyl ether

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study. Biodegradation study, adverse effects on microorganisms derived from toxicity control

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 301 D including toxicity control

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Allyl 2,3-epoxypropyl ether

EC Number:

203-442-4

EC Name:

Allyl 2,3-epoxypropyl ether

Cas Number:

106-92-3

Molecular formula:

C6H10O2

IUPAC Name:

2-[(prop-2-en-1-yloxy)methyl]oxirane

Details on test material:

- Name of test material (as cited in study report): Allyl glycidyl ether
- Physical state: clear colourless liquid
- Analytical purity: 99.58%
- Lot/batch No.: S22491
- Expiration date of the lot/batch: 14 August 2007
- Stability under test conditions: not indicated
- Storage condition of test material: at room temperature in the dark

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands
- Method of cultivation: Secondary effluent was fitlered through a coarse filter paper, the first 200 mL was discarded. The filtrate was kept aerated until inoculation. 4 mL filtrate of secondary effluent per litre of final volume

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Test conditions

Details on test conditions:

TEST CONDITIONS
- Test temperature: between 21.9 and 22.8 °C
- pH: at test start between 7.4 and 7.5
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 250 - 300 mL BOD bottles with glass stoppers
- Number of culture flasks: 2
- Measuring equipment: WTW inolab Oxi Level 2 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G
- Test performed in open system: no

SAMPLING
- Sampling frequency: samples taken at day 0, 7, 14, 21 and 28
- Sampling method: Direct measurement of oxygen content

Results and discussion

Effect concentrations

Duration:

28 d

Dose descriptor:

EC0

Effect conc.:

1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

Biodegradation

Sample	Concentration	% Biodegradation after
		0	7	14	21	28
Reference substance	2.0 mg/L	0	47	76	71	77
Test substance	1.5 mg/L	0	-11	0	6	9
Toxicity control (Reference substance + test substance)	2.0 mg/L + 1.5 mg/L	0	13	27	23	29

Accroding to OECD guideline 301, in a toxicity control at least 25% degradation have to be observed after 14 days of exposure. If this criterion is fulfilled the test substance is considered to be not inhibitory to activated sludge.