Allyl 2,3-epoxypropyl ether

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable well documented study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

BASF-Test: The substance was injected into the peritoneal cavity. Young adult laboratory mice were purchased from breeder. Usually the source and strain of animals were not documented. Several groups of 10 mice per dose were treated simultaneously with preparations of the test substance in suitable vehicle. The concentrations of these preparations were used to achieve comparable volumes per kg body weight. Group-wise documentation of clinical signs was performed over the 7 day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Allyl glycidyl ether

IUPAC Name:

Allyl glycidyl ether

Details on test material:

- Name of test material (as cited in study report): Allyl glycidyl ether
- Physical state: liquid
- Analytical purity: ca. 65%
- Impurities (identity and concentrations): ca. 30% Allyl-ß-chlorhydrin ether

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean body weight at study initiation:
1600 µl/kg bw: male 33-38 g, female 28-30 g
800 µl/kg bw: male 35-39 g, female 25-34 g
400 µl/kg bw: male 34-38 g, female 26-32 g
320 µl/kg bw: male 25-30 g, female 21-24 g
200 µl/kg bw: male 36-38 g, female 25-32 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: aqueous emulsion with Traganth

Details on exposure:

DOSAGE PREPARATION:
- Stock solutions prepared:
2% for the 200 µl/kg bw dose group;
4% for the 320 and 400 µl/kg bw dose group;
8% for the 800 µl/kg bw dose group;
20% for the 1600 µl/kg bw dose group

- Dose volume applied:
10 ml/kg bw of the 2% stock solution for the 200, 400 and 800 mg/kg bw dose group,
8 ml/kg bw of the 16% stock solution for the 320 and 1600 mg/kg bw dose group.

Doses:

200, 320, 400, 800 and 1600 µl/kg bw (corresponds to 194, 310, 388, 776 and 1552 mg/kg bw, respectively)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: mortality and clinical signs of toxicity were observed several times on the application day and thereafter once each working day, body weights were only recorded at the beginning of the study
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 339 mg/kg bw

Mortality:

see free text

Clinical signs:

1600 µl/kg bw: immediately after administration, staggering, convulsions, dyspnea and opisthotonus were observed. Five minutes later, narcosis state was noted. All animals died after 1 h.
800 µl/kg bw: immediately after administration, staggering, convulsions and dyspnea were observed. Two minutes later, abdominal-lateral position, total apathy, closed eyes were noted. All animals died after 4 h.
400 µl/kg bw: immediately after administration, staggering, convulsions, dyspnea, closed eyes, slight jerk and tremors, watery secretions from eyes were observed. Three days later, intermittent respiration and ruffled-fur coat were noted in 2 surviving animals. Four days after injection, no symptoms were observed in the surviving animals.
320-200 µl/kg bw: immediately after administration, slight staggering, forced respiration, closed eyes, sunken flank, watery secretions from the eyes were observed. One day later, accelerated respiration and ruffled-fur coat were noted in the surviving animals. Two-four days after injection, no symptoms were observed in the surviving animals.

Body weight:

not reported

Gross pathology:

Mucus and blood filled swollen intestines, slightly brightened liver

Any other information on results incl. tables

Dose level (µl/kg bw)	Observation Time
	1 h	24 h	48 h	7 days
1600	10/10	10/10	10/10	10/10
800	0/10	10/10	10/10	10/10
400	0/10	8/10	8/10	8/10
320	0/10	3/10	3/10	3/10
200	0/10	0/10	0/10	0/10

LD50 = 350 µl/kg bw = equivalent to ca. 339 mg/kg bw (Density: 0.9698 mg/ml)