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EC number: 204-798-3 | CAS number: 126-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- analytical verification of the test substance is missing, not performed according to GLP and OECD guidelines,
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38419, Part9
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- Cremophor max.5 mg/L
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 86.81
- Source: Algensammlung Göttingen - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.3 °C
- pH:
- 7.3 - 8.1
- Nominal and measured concentrations:
- Test concentrations (nominal): 1, 2.5, 5, 10, 25, 50 mg/L
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 34.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % CL 12.3 - 95.0 mg/L
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 18200055
- Expiration date of the lot/batch: 30 June 2020
- Purity: 98.3 %
- Analytical monitoring:
- yes
- Details on sampling:
- A stock solution with a nominal concentration of 100 mg/L was freshly prepared with dilution water. The dispersion was stirred at 1100 rpm at room temperature for ca. 15 minutes until the solution was visually clear. The stock solution was checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative. Out of the stock solution six concentration levels were tested in a geometrical series with a dilution factor of √10.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding an appropriate amount of the test item (100 mg/L) to dilution water. The dispersion was stirred at 1100 rpm at room temperature for ca. 15 minutes until the solution was visually clear. The stock solution was checked via laser beam (Tyndall effect) for undissolved test item. The Tyndall effect was negative. The stock solution and a further 5 test item concentrations were prepared by diluting the stock solution accordingly.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Dunstaffnage Marine Laboratory, Dunbeg, OBAN; Argyll PA37 1QA, Scotland, UK
- Method of cultivation: Fresh stocks are prepared every month on Z-Agar. Light intensity amounts to 2590 - 5180 lux corresponding to 35 - 70 µE m-2 s-1 for 24 hours per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 38 mg/L as CaCO3
- Test temperature:
- 22 - 23 °C
- pH:
- 7.76 - 8.05 (test start)
8.00 - 9.40 (test end) - Conductivity:
- 141 µS/cm
- Nominal and measured concentrations:
- Nominal: 0.316, 1.0, 3.16, 10, 31.6 and 100 mg/L
Measured (0h): 0.312, 1.18, 3.15, 10.3, 33.3 and 102 mg/L
Measured (72h): 0.296, 0.976, 2.92, 9.32, 27.4 and 95.1 mg/L
measured > 80% nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile Erlenmeyer flasks, volume: 250 mL, sealed with cotton wool plugs with 100 mL test solution
- Initial cells density: 6442 cells/mL
- Control end cells density: 1540086 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guidelines
- Total organic carbon: < LOQ
- Alkalinity: 1 mmol/L
- Conductivity: 141 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH-value at the start of the exposure was measured out of one additional replicate of the test item concentrations and the control. At the end of the exposure it was measured from pooled replicates of the test item concentrations and control, respectively. The room temperature was measured continuously. Light intensity was measured prior to the start of the exposure.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24h light
- Light intensity and quality: 5281 - 6999 lx
EFFECT PARAMETERS MEASURED: Cell density was measured daily via Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal. Self-fluorescence was observed in the preliminary range finding test at the concentration of 120 mg/L. A separate replicate of each test item concentration was prepared without algae and treated like the other test replicates. The self-fluorescence of the measuring points between 24 and 72 hours was taken into account in the evaluation. Microscopic evaluation of the cells at the start and end of the exposure was carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and aggregation of algae cells. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: analytically verified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: analytically verified
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Unusual cell shape: no
- Colour differences: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.799 mg/L
- validity range: 0.753 ± 0.544 - Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance showed a 72h-EC50 of 14.3 and a 72h-EC10 of 10.4 mg/L. Thus, the test substance is harmful to aquatic invertebrates.
Referenceopen allclose all
growth rate:
EC10 (72h): 24.6 mg/L (95 % CL: 10.2 - 59.8 mg/L)
EC10 (96h): 13.3 mg/L (95 % CL: 5.5 - 31.8 mg/L)
EC50 (72h): 34.1 mg/L (95 % CL: 12.3 - 95.0 mg/L)
EC50 (96h): 18.4 mg/L (95 % CL: 7.7 - 43.9 mg/L)
biomass:
EC10 (72h): 23.9 mg/L (95 % CL: 5.0 - 112.3 mg/L)
EC10 (96h): 22.46 mg/L (95 % CL: 4.4 - 114.3 mg/L)
EC50 (72h): 33.2 mg/L (95 % CL: 5.7 - 192.2 mg/L)
EC50 (96h): 30.5 mg/L (95 % CL: 5.3 - 176.9mg/L)
Description of key information
EC50(72h) = 14.3 mg/L (nominal, analytically verified) for growth rate inhibition of Pseudokirchneriella subcapitata (OECD 201)
EC10(72h) = 10.4 mg/L (nominal, analytically verified) for growth rate inhibition of Pseudokirchneriella subcapitata (OECD 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 14.3 mg/L
- EC10 or NOEC for freshwater algae:
- 10.4 mg/L
Additional information
Two studies are available investigating the toxicity of triisubutyl phosphate to freshwater algae. However, one of the studies (BASF 1989) was considered to be not reliable due to methodological shortcomings (missing analytical verification of test concentrations, not performed according to GLP and current OECD guidelines). Thus, a new test was performed according to current quality criteria. This key study was performed according to GLP and OECD giudeline 201 using Pseudokirchneriella subcapitata as test organism (Herrmann 2019). Analytical verification of the test concentrations at the beginning and the end of the test showed > 80% of nominal at any measurement. All validity criteria of the test are fulfilled. The 72h-EC50 is determined to be 14.3 mg/L and the 72h-EC10 is determined to be 10.4 mg/L based on nominal concentrations.
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