Triisobutyl phosphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OPP 81-1 (Acute Oral Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: TSCA

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Triisobutyl phosphate

EC Number:

204-798-3

EC Name:

Triisobutyl phosphate

Cas Number:

126-71-6

Molecular formula:

C12H27O4P

IUPAC Name:

triisobutyl phosphate

Specific details on test material used for the study:

- Name of test material: Tri-isobutylphosphate
- Physical state: clear liquid
- Analytical purity: 99.7%
- Lot/batch No.: NBP 3983659
- Storage condition of test material: room temperature

Test animals

Species:

rat

Strain:

other: CD (Sprague-Dawley derived)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc. Wilmington, Massachusetts, 01887
- Age at study initiation: 9-12 weeeks
- Weight at study initiation: males: 274-339 grams. Females: 224-242 grams
- Fasting period before study: approximately 18 hours
- Housing: Group-housed (six/cage) during equilibration. Individually housed during study. Cages: Suspended, stainless steel with wire mesh bottoms.
- Diet (ad libitum): Purina Laboratory Chow, #5001
- Water (ad libitum): Automatic watering system. Municipal water supply (Elizabethtown Water Co.)
- Acclimation period: 22 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19.4-24.4°C
- Humidity (%): 30-70%
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12 hrs light, 12 hrs dark

IN-LIFE DATES: not specified

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

VEHICLE
Not applicable

MAXIMUM DOSE VOLUME APPLIED:
5.2 mL/kg

DOSAGE PREPARATION:
No preparation was necessary

CLASS METHOD
not applicable

Doses:

5000 mg/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Viability check: twice daily
Observations of Pharmacologic and Toxicologic Signs: App. 1, 2, and 4 hours after dosing and daily thereafter for 14 days.
Body weights: Pre-fast, Post-fast (just prior to dosing; weights used for calcuulation of doses). Day 7 and 14.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology

Statistics:

Not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

One female was found dead on day 1; all five males and four of the five females survived throughout the study.

Clinical signs:

other: Signs seen on the day of dosing in several animals included oral discharge, wet rales, urinary staining, fecal staining, unthrifty coat, abdominal griping and hypoactivity. Signs seen on the day after dosing included dry rales, hypoactivity and evidence o

Gross pathology:

Examinations of the animal which was found dead revealed a variety of changes, primarily in the lungs and gastrointestinal tract. Changes seen in the intestine were suggestive of an irritant effect (the presence of red fluid), and apparent test material was found in the stomach. Except for two animals which exhibited dilated renal pelves and/or a renal pelvis containing white fluid, changes in animals killed after 14 days were similar to those seen in control animals in the laboratory killed by carbon dioxide inhalation.