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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tin tetrachloride
EC Number:
231-588-9
EC Name:
Tin tetrachloride
Cas Number:
7646-78-8
Molecular formula:
Cl4Sn
IUPAC Name:
tin tetrachloride
Details on test material:
- Name of test material (as cited in study report): Zinntetrachlorid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: <= 1g fish / L water


OTHER TEST CONDITIONS
- Adjustment of pH: 8.3
-Temperature: 22+/-1 °C
- Photoperiod: 12h/12h
- Light intensity: ca. 700 Lux
- Water Hardness: 2.1-2.5 mmol CaMg/L
-Oxygen: >= 80% of saturation value


TEST CONCENTRATIONS
0 and 100 mg/L nominal
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
BIOLOGICAL RESULTS
No mortality in the control and the only test concentration of 1000 mg/L. 3 hours after the start of the test all fishes in the test concentration showed different behavior. Symptoms were reversible within the next 3 hours.

ANALYTICAL RESULTS
Concentration < LOD (1.1 mg/L)
Tin tetrachloride precipitated after hydrolysis as Tin(IV)oxide and sedimented

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Tin tetrachloride did not induce acute toxic effects in Brachydanio rerio at a concentration of 1000 mg/L.
Executive summary:

The effects of Tin tetrachloride on Brachydanio rerio were investigated in an acute fish toxicity test with static desing at a limit dose of 1000 mg/L according to OECD 201 under GLP. The control was valid. Tin tetrachloride did not induce acute toxic effects in Brachydanio rerio at a concentration of 1000 mg/L.

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