Glycerol trinitrate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1987-March 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets National standards method with acceptable resctrictions.

Qualifier:

according to guideline

Guideline:

other: ASTM Designation E 729-80 (ASTM, 1980)

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- The stability of the NG stock solution were periodically checked during storage to assure that no decomposition occured. Standard solutions of NG were prepared by dilution of the stock solution freshly prepared each day of analysis. Standard solutions with concentrations of 10.0, 5.0, 2.5, and 1.0 µl/L were used.- Aqueous samples from bioassays were injected directly into HPLC after filtration to remove particles > 0,45 µm.In the cases where samples could not be analysed immediately following filtration, the filtered samples were stored at 4°C in amber glass vials fitted with Teflon-lined caps and analyzedwithin 24 h from the time the samples were originally taken from the test aquaria.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION - Basic solution (the shipment): 1 g NG dissolved in JHU/APL well water. NG solutions were keep in the dark at 4°C in glass bottles.- NG stock solutions were prepared by adding appropriate amounts of the material to aerated JHU/APL well water and stirred for 4 to 8 h. No NG stock solutions were heated during preparation or filtering at 0.45 µm. All stock solutions were prepared in amber glass containers.

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

TEST ORGANISM- Common name:Cladoceran- Source: The Center for Lake Superior Environmental Studies, University of Wisconsin-Superior- Age at study initiation (mean and range, SD): neonate (< 6 h)- Feeding during test- Food type: mixture of Cerophyl® (Cerophyl Laboratories, Inc. Kansas CIty, MO) and the green alga Selenastrum capricornutum, added to the daphnid culture to achieve final concentrations of 120 µg Cerophyl®/mL and Selenastrum capricornutum 6.7 x 100000 cells/mL.- Frequency: at the beginning of the test and at the 24-h renewal period during the testACCLIMATION- Acclimation conditions: 16-h light:8-h dark photoperiod (fluorescent lights, 60-85 foot candles at the surface of the culture vessels).- The stock culture was maintained in 600 m glass beakers, containing 400 m of JHU/APL deep well water, enriched with 2 µg Se (as Na2SeO3)/L as recommended by Winner (1989), and maintained at 25 ± 1°C.- Type and amount of food: mixture of Cerophyl® (Cerophyl Laboratories, Inc. Kansas CIty, MO) and the green alga Selenastrum capricornutum, added to the daphnid culture to achieve final concentrations of 120 µg Cerophyl®/mL and Selenastrum capricornutum 6.7 x 100000 cells/mL.- Feeding frequency: up to the initiation of test

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

164 mg/L (as CaCO3)

Test temperature:

25 +/- 1°C

pH:

8.5

Dissolved oxygen:

8.1 mg/L

Nominal and measured concentrations:

Nominal concentration: 5.8, 9.7, 16, 27, 45 mg/LMeasured concentration (mean): 5.48, 9.45, 15.53, 26.98, 44.8 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: 0.05 L glass beaker- Fill volume: 0.03 L- Volume addition per day: 1 (mean volume addition per day for the complete exposure period)- Renewal rate of test solution (frequency/flow rate): renewed at 24 h- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Non-chlorinated deep well located at The Johns Hopkins University Applied Physics Laboratory (JHU/APL) in Shady Side, MD.- Total organic carbon: 19 mg/L- Metals: Sb, As, Be, Pb, se, Tl < 0.005 mg/L, Cd < 0.001 mg/L, Cr < 0.05 mg/L, Cu < 0.02 mg/L, Hg < 0.0002 mg/L, Ni < 0.2 mg/L, Ag <0.01 mg/L, Zn < 0.04 mg/L- Alkalinity:159 mg CaCO3/L- Conductivity: 409 µmhos/cm- The water used in was analyzed for 43 Priority Pollutants, eight nonpriority, but "Hazardous" substances, 26 pesticides, and 13 metals. None of them were detected with detection limits of 2, 2, 0.1 µg/, and <0.2 - <0.0002 mg/, respectively.- Intervals of water quality measurement: A comprehensive chemical analysis of the well water was conducted two times during the study. The analyses were separated by approx. 18 months.OTHER TEST CONDITIONS- Photoperiod: 16-h light:8-h dark - Light intensity: fluorescent lights, 60-85 foot candles at the surface of the test chambersEFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality at 24 and 48 hrsTEST CONCENTRATIONS- Concentrations for the determinative evaluations were set with two objectives: 1) a geometric series of five concentrations with a dilution factor of 0.6 and 2) two of the five concentrations to produce approximately 84% survival and 84% death, respectively.

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

17.83 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 16.48-19.51 mg/L

Reported statistics and error estimates:

The LC50 value was determined by the moving average angle method, using an EPA statistical program (Stephan, 1978).

Conclusions:

The 48 hr. LC50 of TNG for C. dubia was 17.83 mg/; 95% C.L. = 16.48 – 19.51 mg/L.

Description of key information

The contact of the nitroglycerine with the marine water is impossible in normal conditions of its production and using of explosives.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

17.83 mg/L