Castor oil, ethoxylated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization study of test chemical on Daphnia magna in a static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2

pH:

7.2

Nominal and measured concentrations:

1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Other details not known

Table 1: Immobility

Nominal concentration (mg/l)	No of daphnids	Immobility
		0 hrs	24 hrs	48 hrs
Control	10	-	-	-
1.562	10	-	-	-
3.125	10	-	-	-
6.25	10	-	-	-
12.5	10	-	-	-
25	10	-	-	-
Any        Abnormal Behaviour (if any)		No effects observed	No effects observed	No effects observed

Table 2: pH and oxygen content:
		Nominal	Number of	pH(mg/L)
		Concentration	daphnids	0h	48h
		(mg/l)
		Control	10	7.2	7.3	7.4
		1.562	10	7.3	7.4	7.5
		3.125	10	7.3	7.4	7.6
		6.25	10	7.4	7.5	7.6
		12.5	10	7.4	7.6	7.7
		25	10	7.5	7.6	7.7
		Nominal	Number of DO(mg/L)
		Concentration	daphnids	0h	48h
		(mg/l)
		Control	10	6.8	6.7	6.7
		1.562	10	6.8	6.5	6.5
		3.125	10	6.8	6.5	6.5
		6.25	10	6.8	6.4	6.4
		12.5	10	6.8	6.4	6.4
Test temperature		25	10	6.8	6.4	6.4
		Nominal	Number of Temparature
		Concentration	daphnids	0h	48h
		(mg/l)
		Control	10	21.9		21.7
		1.562	10	21.9		21.7
		3.125	10	21.9		21.7
		6.25	10	21.9		21.7
		12.5	10	21.9		21.7
		25	10	21.9		21.7

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l.

Executive summary:

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

Description of key information

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

25 mg/L

Additional information

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.