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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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toxicity to soil macroorganisms except arthropods: long-term
Data waiving:
study technically not feasible
Justification for data waiving:

Description of key information

Key value for chemical safety assessment

Additional information

Stability study using the related substance octamethyltrisiloxane (L3, CAS 107-51-7) under OECD TG 222 conditions without test organisms:

A stability/recovery test was conducted in preparation for terrestrial ecotoxicology studies with the related substance L3. Vi2-L2 and L3 are members of the Siloxane Category of compounds, and have high vapour pressures (1700 Pa and 530 Pa), high log Kow (5.4 and 6.60), high log Koc (3.2 and 4.3) and low water solubility (0.207 mg/l and 0.034 mg/l). In addition, the substances have slow hydrolysis rates (t1/2 = 140 h and 329 h at pH 7 and 25°C). In the context of terrestrial toxicity, L3 is more likely to remain in the soil as the parent substance during the terrestrial toxicity studies than Vi2-L2 due to the slower hydrolysis rate, lower vapour pressure and higher log Kocof L3. Therefore it is considered valid to read-across the results of the soil stability study with L3.

The study demonstrated a method of introducing neat 14C-octamethyltrisiloxane (14C-L3) into natural soil with subsequent mixing to distribute the test article throughout the soil uniformly. 

The second phase of the study investigated the stability of 14C-L3 in the same soil under conditions representative of those used for the OECD TG 222 Earthworm Acute Toxicity and Reproduction Test. The system was partially open to allow for respiration during a planned future toxicity experiment. 

Effect of headspace on loss of test material during dosing of soil was examined. The main experiment test vessel was selected in order to minimise headspace as much as possible, while still allowing for enough tumbling to ensure homogeneity. However, even the container with the best recovery from the initial recovery test only observed an average recovery of 69.7% (or 22.9 mg/kg soil (d.w.)) after overnight mixing following dosing.

Measures were taken to avoid loss of test substance through volatilisation during the homogeneity experiment. These included:

- fitting the test vessel jar with a polytetrafluoroethylene (PTFE) lined lid and covering the threads of the jar with PTFE tape;

- spiking the soil with a calibrated gastight syringe;

- spiking with a concentration 34% above the saturation concentration, to attempt to account for test substance losses;

- taping the lid closed to avoid inadvertent opening.

The stability experiment was then carried out using the spiked soil from the homogeneity experiment, which was divided into five beakers. Each beaker had 10 mL of headspace and was covered with plastic film having five small holes (approximately 3 mm in diameter) to allow for air exchange. The plastic film was secured with rubber bands. 

By day 3 in the stability experiment, only 6.3% of the initially observed radioactivity was detected, and sampling was stopped. However, the average of the homogeneity determination was taken as the initial concentration for each beaker in the stability test. The soil was not analysed again after dividing into the beakers, therefore the potential loss from moving the test soil from the container used in the homogeneity experiment to the 5 test beakers used in the stability study was not determined.

A single peak was observed consistent with 14C-L3, showing that the primary mechanism of loss in the initial recovery experiment was volatilisation.

The absence of degradation products in the vast majority of samples, coupled with the rapid loss of 14C activity, shows that the primary mechanism of test article loss was volatilisation of 14C-L3 from the simulated OECD TG 222 test setup.