Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10 July 1984 to 15 August 1984
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
Test substance; FAT 20306/B
Appearance: red powder
Purity: active ingredient
Solubility: 50 g/l water
Storage: room temperature
Stability: 30 years (2014)
Stock solution: 200 mg FAT 20306/B were dissolved in water and made up to 1000 ml with water.
Analytical monitoring:
yes
Details on sampling:
The test substance was dissolved at all test concentrations. Samples for analysis were taken after 0 h and 24 h exposure.
Vehicle:
no
Details on test solutions:
Test concentration: 18, 32, 58, 100, 180 mg/l (nominal)
Calculated amounts of stock solution to produce the desired test concentrations were given into the water and homogeneously distributed.
Test organisms (species):
Daphnia magna
Details on test organisms:
Strain: DAPHNIA MAGNA STRAUS 1820
Source: CIBA-GEIGY Ltd., Facilities, Basle
Breeding: Parthenogenetic reproduction. Feed: Suspension of green algae supplemented by trout chow and/or powder milk and/or yeast; in such quantities that food is consumed after 24 hours.
Pretreatment: 24 hours before the start of the test reproductive daphnia are separated from the young by sieving through an 800 micrometer sieves. This operation is repeated immediately before the start and the young daphnia (0 - 24 h old) are retained for the test.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
240 mg CaCO3/l
Test temperature:
20 - 21 °C
pH:
7.8 - 7.9
Dissolved oxygen:
8.2 - 8.9 mg/l; 93 - 99 % of saturation
Salinity:
no data
Nominal and measured concentrations:
nominal: 18, 32, 58, 100, 180 mg/l
measured: 91 - 99 % of nominal
Details on test conditions:
Vessels: 20 ml test tubes, covered with steel caps (10 ml solution)
Water: Reconstituted water produced by dissolving:
65 mg NaHC03
294 mg CaCl2•2H2O
123 mg MgS04•7H2O
6 mg KCl
in 1000 ml bidistilled water.
The water was aerated with clean air for at least 24 h before use
Controls: blank: water
Number of daphnids: 20 daphnia/conc. and control. (4 replicates of 5 daphnia)
Feeding: None during the test,
Aeration: none
Duration: 24 hours
Lighting: 16 hours fluorescent light daily, approx. 2000 lux
Measurements: (pH, O2, temperature) at the beginning and at the end of the test.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
49 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
calculated
Remarks on result:
other: 41-57 mg/l
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
54 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
graphically determined
Details on results:
Controls : Immobilization blank 0 %
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
The EC50 values were calculated according to J.EOKSON, JA5A 40(1953), 569-599, EC50 was graphically determined on gausso-logarithnic probability paper.

 Immobilization:

Concentrations Noninal (mg/L)

Immobilization after 24 hours

1

2

3

4

total

%

Blank

0

0

0

0

0

0

18

0

0

0

0

0

0

32

2

2

1

0

5

25

58

4

3

3

4

14

70

100

4

5

4

5

18

90

180

4

5

5

5

19

95

Validity criteria fulfilled:
yes
Conclusions:
The calculated and graphically determined 24 h EC50 of FAT 20306/B in acute daphnia test is 49 and 54 mg/L, respectively.
Executive summary:

The test was performed to determine the EC50 (concentration where 50 % of the daphnids are immobilized) to DAPHNIA MAGNA STRAUS 1820 during 24 hours test period according to OECD test method 202. No immobilisation was found at 18 mg/L concentration group only. Based on the study results, the calculated EC50 (24h) of was 49 mg/L (confidence limit of 41-57 mg/L). The graphically determined EC50 (24 h) was 54 mg/L.

Description of key information

 The calculated and graphically determined 24 h EC50 of FAT 20306/B in acute daphnia test is 49 and 54 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
49 mg/L

Additional information

The test was performed to determine the EC50 (concentration where 50 % of the daphnia are immobilized) to DAPHNIA MAGNA STRAUS 1820 during 24 hours test period according to OECD test method 202. No immobilisation was found at 18 mg/L concentration group only. Based on the study results, the calculated EC50 (24h) of was 49 mg/L (confidence limit of 41-57 mg/L). The graphically determined EC50 (24 h) was 54 mg/L.