[2-(acryloyloxy)ethyl]trimethylammonium chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05/12/1994-08/12/1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

EC Number:

256-176-6

EC Name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

Cas Number:

44992-01-0

Molecular formula:

C8H16NO2.Cl

IUPAC Name:

Trimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride

Details on test material:

- Name of test material: ADAM MECL
- Physical state: colourless, slightly viscous liquid
- Composition of test material, percentage of components: 80 % aqueous solution
- Lot/batch No.: 94097 November 15, 1994
- Expiration date of the lot/batch: 2 months
- Storage condition of test material: in darkness at room temperature
- Packaging: 250ml plastic flask

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Test water

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CHODAT 86-81 SAG
- Source : Aquarium de Trouville, France
- Age of inoculum (at test initiation): approximately 72-hour
- Method of cultivation: axenic subcultures made at regular intervals

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

No

Test conditions

Hardness:

< 90 mg CaCO3 per litre of culture medium

Test temperature:

23°C ( room temperature)

pH:

7.11 (readjusted with HCl 0.1N)

Nominal and measured concentrations:

0; 1; 10; 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: no data
- Material, size, headspace, fill volume: 50 mL
- Aeration: no
- Type of flow-through: static
- Initial cells density: 10,000mg/L
- No. of vessels per concentration: 3
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: the test medium was a mixture of culture medium, algal inoculum and the test substance at selected concentrations (apart from the control), to form a final volume of 50 mL per task flask.
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: 7.11 readjusted with HCl 0.1N
- Photoperiod: continuous lighting
- Light intensity and quality: artifcial continuous white lightening, providing from 6000 lux to 6100 lux
TEST CONCENTRATIONS
- Test concentrations: 0; 1; 10; 100 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC100

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 6

Duration:

72

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Any other information on results incl. tables

GROWTH INHIBITION
	Algal Concentration
Nominal Concentration (mg/L)	Start	24 hours	48 hours	72 hours	% growth inhibition
0	10,000	188,340	2,248,344	13,850,004	0
1	10,000	380,004	1,923,336	12,283,332	11
10	10,000	230,004	806,676	2,933,340	79
100	10,000	180,000	306,672	300,012	98
GROWTH RATE INHIBITION
Nominal Concentration (mg/L)	Growth rate			% growth rate inhibition
0	0.0612			–
1	0.0604			1
10	0.0384			37
100	0.0046			93

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the EC50 at 72 hours for both growth rate and biomass were situated between 10 and 100 mg/l. A No Observed Effect Level was not identified. The Lowest Observed Effect Level was 1 mg/l.