Registration Dossier
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EC number: 202-716-0 | CAS number: 98-95-3
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, acceptable for assessment.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Comparative toxicity of ten organic chemicals to four earthworm species
- Author:
- Neuhauser et al.
- Year:
- 1 986
- Bibliographic source:
- Comp. Pharmacol. Toxicol. 83: 197-200
- Reference Type:
- secondary source
- Title:
- European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
- Author:
- European Chemicals Bureau
- Year:
- 2 007
- Bibliographic source:
- 3rd Priority List, Volume: 77
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Development of a standardized toxicity laboratory method for assessing the toxicity of chemical substances to earthworms. Report EUR 8714EN. Com- Acknowledgement-This work was supported by a grant mission of theCommission of the European Community.
- GLP compliance:
- no
Test material
- Reference substance name:
- Nitrobenzene
- EC Number:
- 202-716-0
- EC Name:
- Nitrobenzene
- Cas Number:
- 98-95-3
- Molecular formula:
- C6H5NO2
- IUPAC Name:
- nitrobenzene
- Details on test material:
- - Analytical purity: >98%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone or chloroform (if used!)
Test organisms
- Test organisms (species):
- other: Eisenia fetida, Allolobophora tuberculata, Eudrilus eugeniae, Perionyx excavatus
- Animal group:
- annelids
- Details on test organisms:
- - Housing conditions: at room temperature (20 -22 °C) on a consistent organic food source (horse manure)
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20 °C
- pH:
- 6.0 ± 0.5
- Moisture:
- 35% dry wt
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): covered glass dishes, 6.5 cm high and 12.5 cm in diameter
- Amount of soil or substrate: 400g dry wt
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE
Artificial soil: consisted by weight of 10% finely ground sphagnum peat, 20% kaolinite clay, 69% fine sand and 1% pulverized calcium carbonate.
Water content: 35% of the dry wt of the four components
EFFECT PARAMETERS MEASURED: mortality after 14 days
TEST CONCENTRATIONS
- Range finding study: yes - Nominal and measured concentrations:
- - Five chemical concentrations
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 319 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Species: Eisenia fetida
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 226 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Species: Eudrilus eugeniae
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 343 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Species: Perionyx excavatus
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 362 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Species: Allolobophora tuberculata
- Reported statistics and error estimates:
- LC50 values were calculated using the method of Litchfield and Wilcoxon, 1949
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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