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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Study was not performed according to guideline standards, without detailed documentation of results (just one figure presented)

Data source

Reference
Reference Type:
publication
Title:
Characterization of the tumor-promoting activity of m-chloroperoxybenzoic acid in SENCAR mouse skin and its inhibition by gallotannin, oligomeric proanthocyanidin, and their monomeric units
Author:
Chen G, Perchellet EM, Gao XM, Johnson FK, Davis AW, Newell SW, Hemingway RW, Bottari V, Perchellet JP
Year:
1996
Bibliographic source:
Int. J. Oncology 8: 197-206

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
16 h after two topical applications of DCP within 72 h DNA synthesis was measured by rate of incorporation of [methyl-3H]-thymidine
GLP compliance:
no
Type of assay:
unscheduled DNA synthesis

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
Sencar
Sex:
female
Details on test animals and environmental conditions:
5 weeks old,
from Harlan Sprague Dawly Inc., Indianapolis, IN

Administration / exposure

Route of administration:
dermal
Vehicle:
Acetone
Details on exposure:
substance was dissolve in acetone and applied on the back
Frequency of treatment:
twice within 72 h
Post exposure period:
16 h
Doses / concentrations
Remarks:
Doses / Concentrations:
2.5 mg
Basis:
nominal conc.
No. of animals per sex per dose:
4
Control animals:
yes, concurrent vehicle

Results and discussion

Any other information on results incl. tables

No increase in DNA synthesis was observed after two topical applications of 2.5 mg dicumylperoxide.

Control showed a DNA synthesis of approx. 50 cpm/µg DNA. After application of dicumylperoxide a value of 45 cpm/µg DNA was obtained (all values read from figure).

Applicant's summary and conclusion