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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: screening test, other
Remarks:
inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, basic information available by the test report
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No surrogate or analogue material was used.
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
- Source of activated sludge: aeration tank of the sewage treatment plant "Aquanet S.A., Centralna Oczyszczalnia, Scieków ul. Gdynska 1, Kozieglowy
- Date of collection: 2010-03-15
- Pretreatment: Washing twice, centrifugation for separation of the sludge
- Concentration of activated sludge in reaction mixture: 0.3 g ss/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 20 - 25 °C
- Aeration of dilution water: yes
- Suspended solids concentration: 0.3 g ss/L medium
- Continuous darkness: yes

TEST SYSTEM
- Test suspension: mineral medium, test substance and inoculum
- Procedure control: mineral medium, reference substance and inoculum
- Inoculum blank: mineral medium and inoculum
- Toxicity control: mineral medium, test substance, reference substance and inoculum
- Test volume: 1000 mL
- Chemical analysis: Determination of dissolved total organic carbon (DOC)
- Test apparatus: Spectrophotometer CADAS Dr. Lange

SAMPLING
- Sampling frequency: daily
Reference substance:
other: Sodium benzoate
Preliminary study:
Not applicable.
Test performance:
Neither unusual observations during test nor any other information affecting results are reported.
Parameter:
% degradation (DOC removal)
Value:
1.5
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
3.2
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
5.3
Sampling time:
21 d
Parameter:
% degradation (DOC removal)
Value:
7.1
Sampling time:
27 d
Parameter:
% degradation (DOC removal)
Value:
8.2
Sampling time:
28 d
Details on results:
No toxic effects to bacteria were observed.
Results with reference substance:
The reference compound reached the level of greater or equal to 70 % degradation within 14 days, since at this time point, 98 % degradation has had occurred.
Validity criteria fulfilled:
yes
Remarks:
Validity criteria of the applied guideline were met.
Interpretation of results:
not inherently biodegradable
Conclusions:
The study report describes about a valid biodegradation study according to OECD Guideline 302 B (Zahn-Wellens/EMPA Test). According to the obtained degradation of 8.2 % after 28 days, the test substance is considered as not inherently biodegradable.
Executive summary:

The biodegradation potential of Fe(III)HBED was investigated according to OECD Guideline 302 B (Chojnacka, 2010). Activated sludge was used as inoculum and mixed with the test substance and mineral medium in 2 parallels. Further flasks were the procedure control (with the reference substance sodium benzoate), the inoculum blanks (each in 2 parallels) and one toxicity control (containing mineral medium, test substance, reference substance and inoculum). The suspended solid concentration was 0.3 g ss/L in each flask. The mixtures were agitated and aerated at 20 - 25 °C in the dark for a period of 28 days. The biodegradation process is monitored by determination of dissolved total organic carbon (DOC) with a spectrophotometer in filtered samples taken in daily intervals. The ratio of eliminated DOC, corrected for blank after each time interval, to the initial DOC value is expressed as the % biodegradation at the sampling time. The reference item attained 98 % degradation already after 14 days incubation, thereby fulfilling the validity criteria of the applied guideline, concluding that more or equal to 70 % degradation should have been occurred after 14 days. The test substance showed no toxic effects towards the used bacteria, since the toxicity control attained 55 % degradation after 28 days. The DOC content of the blank was below 10 mg/L at the start of the experiment. The test substance attained 3.2 % degradation after 14 days and 8.2 % degradation after 28 days, respectively. The test substance cannot be regarded as inherently biodegradable.

Description of key information

OECD 302B, Zahn-Wellens Test: 8.2 % degradation after 28 days - not inherently biodegradable  

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The biodegradation potential of Fe(Na)HBED was investigated according to OECD Guideline 302 B (Chojnacka, 2010). Activated sludge was used as inoculum and mixed with the test substance and mineral medium in 2 parallels. Further flasks were the procedure control (with the reference substance sodium benzoate), the inoculum blanks (each in 2 parallels) and one toxicity control (containing mineral medium, test substance, reference substance and inoculum). The suspended solid concentration was 0.3 g ss/L in each flask. The mixtures were agitated and aerated at 20 - 25 °C in the dark for a period of 28 days. The biodegradation process is monitored by determination of dissolved total organic carbon (DOC) with a spectrophotometer in filtered samples taken in daily intervals. The ratio of eliminated DOC, corrected for blank after each time interval, to the initial DOC value is expressed as the % biodegradation at the sampling time. The reference item attained 98 % degradation already after 14 days incubation, thereby fulfilling the validity criteria of the applied guideline, concluding that more or equal to 70 % degradation should have been occurred after 14 days. The test substance showed no toxic effects towards the used bacteria, since the toxicity control attained 55 % degradation after 28 days. The DOC content of the blank was below 10 mg/L at the start of the experiment. The test substance attained 3.2 % degradation after 14 days and 8.2 % degradation after 28 days, respectively. The test substance cannot be regarded as inherently biodegradable.