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Reliable results are available from short-term tests with fish, aquatic invertebrates and algae. LL50/EL50 values determined in tests conducted with water-accommodated fractions (WAFs) of TOH were >100, >100 and >100 mg/L for Danio rerio (fish), Daphnia magna (invertebrate) and Desmodesmus subspicatus (algae) respectively. A NOELR of ≥100 was also determined in the test with D. subspicatus.

No measured data are available for long-term toxicity of TOH to fish or invertebrates. Data-waivers are considered to be appropriate for this endpoint on the grounds that calculation of PNECs for the aquatic compartment will be based on data for the blocks of constituents rather than on data for the whole substance.

Testing for long-term toxicity to fish will not therefore contribute to the database that is required to complete the assessment of the substance.