Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-04-28 to 2010-06-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Acetic acid,oxo-,sodium salt (FeNaEDDHA)
-Synonym: Acetic acid,oxo-,sodium salt, reaction products with ethylenediamine and phenol,iron sodium salts
- Physical state: Solid/ red, dark
- Analytical purity: 100 % (UVCB)
- Lot/batch No.: 09226BR8
- Expiration date of the lot/batch: 07 Mar 2012
- Storage condition of test material: At ambient temperature under exclusion of light

Analytical monitoring:

yes

Details on sampling:

At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed.
The samples were transported to the Analytical Laboratory on the day of sampling.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was weighed in (119.69 mg) and made up with 1000 mL M4 medium. The mixture was stirred on a magnetic stir plate for about 10 minutes in the dark at 20 ± 2 degree C.
All test solutions were visibly clear red colored over the exposure period.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: Daily
- Food type: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.20 – 3.20 mmol/L CaCO3

Test temperature:

19.5 – 19.9 °C (continuous monitoring)

pH:

7.5 – 8.5

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concnetration: 120 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Aeration: approximately 24 hours prior to use
- Renewal rate of the culture water: Twice weekly
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates with test animals (5 animals per replicate)
- Total No. of animals per concentration: 20


TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 2.20 – 3.20 mmol/L
- Ca/mg ratio: about 4 : 1
- Conductivity: 550 - 650 uS/cm

OTHER TEST CONDITIONS
- Photoperiod/Light intensity: The test was performed in the dark due to the potential light sensitivity of the test substance.

EFFECT PARAMETERS MEASURED: Each test vessel was visually checked for immobilized daphnids after 0, 24 and 48 hours. In addition any abnormal behavior or appearance was documented.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50: The EC50(24h) of the reference substance potassium dichromate was 1.19 mg/L.
- Other: This result is within the range of 0.6 – 2.1 mg/L (ISO 6341) and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress.

Reported statistics and error estimates:

Since the study is designed as a limit test, no statistical analysis was performed.

Number of mobile Daphnids

Nominal concentration [mg/L]	Replicate 1 [h]			Replicate 2 [h]			Replicate 3 [h]			Replicate 4 [h]			∑	∑
	0	24	48	0	24	48	0	24	48	0	24	48	24	48
0 (control)	5	5	5	5	5	5	5	5	5	5	5	5	20	20
120	5	5	5	5	5	5	5	5	5	5	5	5	20	20

Effect concentrations after 24 hours and 48 hours of exposure. The EC values based on nominal concentrations were:

Time [h]	EC0 [mg/L]	EC50 [mg/L]	Confidence limits 95% [mg/L]	EC100 [mg/L]
24	>120	> 120	* – *	> 120
48	>120	> 120	* – *	> 120

*The data were insufficient to determine confidence limits.

Validity criteria fulfilled:

yes

Conclusions:

In conclusion the 48 hour EC50 values for FeNaEDDHA in this acute toxicity study with Daphnia magna were > 120 mg/L based on the nominal concentration of the test substance and > 122 mg/L based on the mean measured concentrations.

Executive summary:

This acute toxicity study investigated the effect of FeNaEDDHA on the immobilisation of Daphnia magna according to the principles of OECD-Guideline 202 and EU method C.2. Juvenile daphnids (younger than 24 hour old) were exposed to one concentration of the test substance and compared to a control. Immobilisation of daphnia is determined after 24 and 48 hours of exposure. The test substance was was tested at 120 mg/L.

The 48 hour EC50 value for FeEDDHA with Daphnia magna was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean measured concentrations. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration for the evaluation of the test substance.

Description of key information

EC50(48h) > 122 mg/L (measured, limit test) for immobilisation of Daphnia magna (OECD 202)

Additional information

In the key study the acute toxic effects of FeNaEDDHA to Daphnia magna were determined according to the principles of OECD guideline 202 and EU method C.2. A limit test with a nominal concentration of 120 mg/L was carry out at a static system. The EC50 for a 48 hour exposure was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean measured concentrations.