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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable with restriction. Not according to GLP nor to specific test guidelines.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Chromosomal aberrations in cultured human leucocytes induced by cadmium sulfide
Author:
Shiraishi Y, Kurahashi H and Yoshida TW
Year:
1972
Bibliographic source:
Proc Jap Acad 48(2): 133-137

Materials and methods

Principles of method if other than guideline:
CA:
-incubation time: 72 hours
-number of cells observed: 50 metaphases per culture
-staining: Giemsa
GLP compliance:
not specified
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
name of test substance: cadmium sulphide

Method

Target gene:
not applicable
Species / strain
Species / strain / cell type:
lymphocytes:
Details on mammalian cell type (if applicable):
not applicable
Additional strain / cell type characteristics:
not applicable
Metabolic activation:
not applicable
Metabolic activation system:
not applicable
Test concentrations with justification for top dose:
6.2x10-2 µg/ml
Vehicle / solvent:
no information
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
no
Positive control substance:
no
Remarks:
none
Details on test system and experimental conditions:
none
Evaluation criteria:
no information
Statistics:
not reported

Results and discussion

Test results
Species / strain:
lymphocytes:
Metabolic activation:
not applicable
Genotoxicity:
positive
Remarks:
CA
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not valid
Untreated negative controls validity:
valid
Positive controls validity:
not specified
Additional information on results:
none
Remarks on result:
other: strain/cell type: human leucocytes
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

duration of treatment (hours)  total No of cells observed  No of cells with chromatid breaks (%)  No of cells with isochromatid breaks (%)  No of cells with translocation (%)  No of cells with dicentric chromosomes (%) 
0 (control)  50 
50  17(34)  2(4)  7(14) 
50  7(14)  14(28)  6(12)  3(6) 

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive

This study reported that Cadmium sulfide (form unspecified) induced chromosomal aberrations in cultured human lymphocyes.
Executive summary:

Shiraishi et al. (1972) reported an increased frequency of chromosome aberrations in human lymphocytes exposed to cadmium sulphide in vitro.