Copper dichloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication report which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test material was administered to groups of five animals by the oral route. The LD50 with its fiducial limit was calculated. Gross behavioural effects were also evaluated following administration of the test material at 1/10 of the LD50 by the oral route.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Copper dichloride

EC Number:

231-210-2

EC Name:

Copper dichloride

Cas Number:

7447-39-4

Molecular formula:

CuCl2

IUPAC Name:

copper dichloride

Details on test material:

- Name of test material (as cited in study report): Cupric chloride.
- Composition of test material, percentage of components: Not stated.
- Lot/batch No.: Not stated.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Wistar albino rats were of either sex and weighed 100 - 125 grams. Test animals were kept in polycarbonate cages at an ambient temperature of 24°C +/- 0.5°C with a 12 hour dark/ 12 hour light cycle. The animals were fed Gold Mohur Hind Lever diet and given water ad libitum.

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

water

Details on oral exposure:

Test material was dissolved ih de-ionised triple glass distilled water and administered orally. The equivalent volume of vehicle only was given to a control group.

Doses:

Doses used for estimation of the LD50 were not stated.
For assessment of gross behavioural effects the test material was administered at 1/10 of the LD50.

No. of animals per sex per dose:

Each group consisted of five animals.

Control animals:

yes

Details on study design:

The study was conducted over a period of 10 - 15 hours.
For the assessment of gross behavioural effects any changes were recorded at 30, 60, 120 and 240 minutes.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

584 mg/kg bw

Based on:

test mat.

Other findings:

Administration of copper dichloride at a concentration of 1/10 LD50 resulted in moderate reductions in spontaneous activity, muscle tone, touch response and respiration.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral LD50 of the test item to the rat was 584 mg/kg bw.
Classification according to Directive 67/548/EEC: Harmful (Xn). R22, Harmful if swallowed.
Classification according to CLP/GHS: Acute Tox. 4, H302: Harmful if swallowed.

Administration of copper dichloride at a concentration of 1/10 LD50 resulted in moderate reductions in spontaneous activity, muscle tone, touch response and respiration.

Executive summary:

A study was conducted to determine the acute oral toxicity of copper dichloride to rats. The potential for effects on gross behaviour was also evaluated following administration of the test material at a concentration equivalent to 1/10 of the LD50. The study was not conducted in accordance with GLP and did not follow an internationally accepted guideline. Nonetheless, the results are considered to be sufficiently robust to allow an assessment of the classification and labelling of the test material to be made. Test material was dissolved in water prior to administration to groups of five animals. Control animals received the vehicle only. The acute oral LD50 of copper dichloride to the rat was reported to be 584 mg/kg bw. On this basis, copper dichloride is classified as Acute Tox. 4, H302: Harmful if swallowed. Copper dichloride administered orally at a concentration 1/10 of the LD50 showed CNS depressant properties (depression of the moderate reductions in spontaneous activity, muscle tone, touch response and respiration).