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EC number: 209-942-9 | CAS number: 598-62-9
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
NOEC =1.3 mg/L mean measured concentration of test substance in test media, OECD 211, EPA OPPTS 850.1300, Priestly & Mullee (2010). Study conducted with MnO.
Key value for chemical safety assessment
Additional information
The effect of the test substance on the survival and reproduction of Ceriodaphnia dubia over an 8 -day period was investigated in a study which was conducted under GLP conditions and partly in accordance with the standardised guidelines OECD 211 and EPA OPPTS 850.1300.
As the test substance is considered to be poorly soluble in water saturated solutions of test substance were prepared for use in the study.
Based an acute toxicity to Daphnia magna study (Harlan Laboratories Ltd Project Number: 2702/0143), Ceriodaphnia dubia were exposed (10 replicates of a single daphnid per group) to an aqueous solution of the test substance over a range of test concentrations of 10, 32 and 100% v/v saturated solution for a period of 8 days.The test solutions were renewed 3 times during the study. The numbers of live and dead adult Ceriodaphnia and young daphnids (live and dead) were determined daily. The Ceriodaphnia were fed daily with an algal suspension and YAT (yeast, alfalfa, trout chow) combination.
Exposure of Ceriodaphnia dubia to the test substance resulted in significant mortalities at the test concentration of 4.1 mg/L as test substance resulting in 50% mortality by Day 8. The 8-day EC50 (immobilisation) value, based on mean measured test concentration, as test substance, for the parental Ceriodaphnia generation (P1), was estimated to be approximately 4.1 mg/L. The 8-day EC50 (reproduction) based on mean measured test concentration, as test substance, was 2.5 mg/L with 95% confidence limits 2.2-2.8 mg/L.
The ''Lowest Observed Effect Concentration'' (LOEC) and the ''No Observed Effect Concentration'' (NOEC) based on mean measured test concentration, as test substance, were 4.1 and 1.3 mg/L, respectively.
Since the study was conducted with manganese oxide, rather than with the registered substance itself, it was assigned a relibality score of 2 according to the criteria of Klimisch (1997).
Use of data on MnO to address data requirements of MnCO3 is considered to be justified on the basis that analogous results were obtained following chronic daphnia, and algae, testing with both substances, both substances display similar TDp results suggesting similar levels of metal release in environmentally relevant waters, and manganese is in the same oxidation state in both substance; furthermore, the anions are not expected to cause aquatic toxicity.
Overall, the NOEC generated from this study (1.3 mg/L test substance) supports the conclusion that the registered substance is not hazardous to the aquatic environment.
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