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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN EN ISO 11348-2
Principles of method if other than guideline:
A suspension of bioluminiscent bacteria is brought into a medium containing a specific concentration of copper slag extracts. After 30 minutes, the inhibition of bioluminiscence is assessed.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
slag, copper smelting
EC Number:
266-968-3
Cas Number:
67711-92-6
IUPAC Name:
slag, copper smelting
Details on test material:
Slow cooled copper slag (stones) with the following chemical composition:
Fe 40.01%
SiO2 32.12 %
Cu 9300 mg/kg
Pb 4150 mg/kg
Zn 25100 mg/kg
As 12 mg/kg
Ni < 5 mg/kg
Co 310 mg/kg
Cd 17 mg/kg
Sn 1545 mg/kg

Sampling and analysis

Details on sampling:
at Tend

Test solutions

Details on test solutions:
Eluates prepared in ratio 1 /10 ( 100 g sample in 1000 ml water ) according to DIN 38414
Concentrations in eluate ( mg/l)
As 0.027
Ba 0.11
Ca 14
Cd <0.0001
Co <0.01
Cr<0.1
Cu 0.02
Hg < 0.0002
Mo 0.12
Ni < 0.01
Pb < 0.027
Sb 0.004
Tl <0.0005
Zn 0.01

Test organisms

Test organisms (species):
Vibrio fisheri

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min

Test conditions

Hardness:
DOC 1.43 mg/l
pH:
9.9

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 80 g/L
Conc. based on:
test mat.
Basis for effect:
other: bioluminescence

Any other information on results incl. tables

Concentration of the eluate in test concentration

Eluate (1-10)

Luminescent bacteria toxicity, GI - value

1

 

*Smallest dilution factor that results in a 20% light emission reduction

EC 20

> 800 ml eluate/L

 

The above values can be converted to toxicity for copper slag by taking into account the amount of slag used to produce the eluate : 100 g slag / 1000 ml water

EC 20

> 80 g copper slag /L

Applicant's summary and conclusion

Conclusions:
An EC20 value of >80 g/L was observed.
Executive summary:

Effect of water extracts of copper slags on the bioluminescence of Vibrio fischeri was assessed during a 30 min experiment. The test was performed according to DIN EN ISO 11348 -2. An EC20 value of >80 g/L was observed.