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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. Range-finding test.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1962

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Range-finding test
Groups of 6 albino rats were exposed to 2-hydroxy-2-methylpopionitrile vapors that were produced by passing a 2.5-l/min-air stream through a
fritted glass disc immersed in 50 ml acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated
explicitly). Exposure period was 4 hours. The observation period was 14 days.
GLP compliance:
no
Remarks:
study performed before implementation of GLP
Test type:
other: Range-finding test
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hydroxy-2-methylpropionitrile
EC Number:
200-909-4
EC Name:
2-hydroxy-2-methylpropionitrile
Cas Number:
75-86-5
Molecular formula:
C4H7NO
IUPAC Name:
2-hydroxy-2-methylpropanenitrile
Details on test material:
- Name of test material (as cited in study report): alpha-Hydroxyisobutyronitrile (no further information)

Test animals

Species:
rat
Strain:
other: Albino
Sex:
not specified
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Details on inhalation exposure:
Rats were epose to acetone cyanohydrin vapors that were produced by passing a 2.5-L/min-air stream through a fritted glass disc immersed in
50 mL acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly).
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly).
No. of animals per sex per dose:
6 animals per dose
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no data

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LCLo
Effect level:
62.5 ppm
Exp. duration:
4 h
Remarks on result:
other: 5 min caused no death, 62.5 ppm (equals 0.223 mg/L) 2/6 rats died after 4 hours
Sex:
not specified
Dose descriptor:
LC100
Effect level:
125 ppm
Exp. duration:
4 h
Remarks on result:
other: 6/6 rats died, 125 ppm (equals 0.446 mg/L (446 mg/m³/4hr))
Mortality:
5 min of exposure:
62.5 ppm: no death (0/6 rats died after 5 min of exposure)
4 hour exposure:
62.5 ppm: 2/6 rats
125 ppm: 6/6 rats

Clinical signs:
other: No other signs of toxicity were reported.
Body weight:
no data
Gross pathology:
no data

Any other information on results incl. tables

No NOEL established.

Applicant's summary and conclusion

Interpretation of results:
Category 2 based on GHS criteria
Remarks:
Migrated information
Conclusions:
Legally binding harmonised classification as given in REGULATION (EC) No 1272/2008 Annex VI Table 3.1 (GHS): Acute tox. 2*; H330 and Table 3.2 (Annex I of Directive 67/548/EEC): T+; R26/27/28.
Executive summary:

Groups of 6 albino rats were exposed to 2-hydroxy-2-methylpopionitrile vapors that were produced by passing a 2.5-l/min-air stream through a fritted glass disc immersed in 50 ml acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly). The observation period was 14 days. After exposure for 4 hours, 2/6 rats were killed at 62.5ppm and 6/6 rats were killed at 125 ppm. No other signs of toxicity were reported.