2-hydroxy-2-methylpropionitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. Range-finding test.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1962

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Range-finding test
Groups of 6 albino rats were exposed to 2-hydroxy-2-methylpopionitrile vapors that were produced by passing a 2.5-l/min-air stream through a
fritted glass disc immersed in 50 ml acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated
explicitly). Exposure period was 4 hours. The observation period was 14 days.

GLP compliance:

no

Remarks:

study performed before implementation of GLP

Test type:

other: Range-finding test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-2-methylpropionitrile

EC Number:

200-909-4

EC Name:

2-hydroxy-2-methylpropionitrile

Cas Number:

75-86-5

Molecular formula:

C4H7NO

IUPAC Name:

2-hydroxy-2-methylpropanenitrile

Details on test material:

- Name of test material (as cited in study report): alpha-Hydroxyisobutyronitrile (no further information)

Test animals

Species:

rat

Strain:

other: Albino

Sex:

not specified

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Details on inhalation exposure:

Rats were epose to acetone cyanohydrin vapors that were produced by passing a 2.5-L/min-air stream through a fritted glass disc immersed in
50 mL acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly).

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly).

No. of animals per sex per dose:

6 animals per dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no data

Results and discussion

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

LCLo

Effect level:

62.5 ppm

Exp. duration:

4 h

Remarks on result:

other: 5 min caused no death, 62.5 ppm (equals 0.223 mg/L) 2/6 rats died after 4 hours

Effect levels 2

Sex:

not specified

Dose descriptor:

LC100

Effect level:

125 ppm

Exp. duration:

4 h

Remarks on result:

other: 6/6 rats died, 125 ppm (equals 0.446 mg/L (446 mg/m³/4hr))

Mortality:

5 min of exposure:
62.5 ppm: no death (0/6 rats died after 5 min of exposure)
4 hour exposure:
62.5 ppm: 2/6 rats
125 ppm: 6/6 rats

Clinical signs:

other: No other signs of toxicity were reported.

Body weight:

no data

Gross pathology:

no data

Any other information on results incl. tables

No NOEL established.

Applicant's summary and conclusion

Interpretation of results:

Category 2 based on GHS criteria

Remarks:

Migrated information

Conclusions:

Legally binding harmonised classification as given in REGULATION (EC) No 1272/2008 Annex VI Table 3.1 (GHS): Acute tox. 2*; H330 and Table 3.2 (Annex I of Directive 67/548/EEC): T+; R26/27/28.

Executive summary:

Groups of 6 albino rats were exposed to 2-hydroxy-2-methylpopionitrile vapors that were produced by passing a 2.5-l/min-air stream through a fritted glass disc immersed in 50 ml acetone cyanohydrin. Doses were logarithmically distributed, differing by a factor of two (doses were not stated explicitly). The observation period was 14 days. After exposure for 4 hours, 2/6 rats were killed at 62.5ppm and 6/6 rats were killed at 125 ppm. No other signs of toxicity were reported.