2-hydroxyethyl acrylate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Study meets generally accepted scientific standards with restrictions due to the limited documentation in the summary report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Principles of method if other than guideline:

BASF test
Aqueous preparations of the test substance were injected into the peritoneal cavity of the mice. Group-wise documentation of clinical signs was performed over the 7-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxyethyl acrylate

EC Number:

212-454-9

EC Name:

2-hydroxyethyl acrylate

Cas Number:

818-61-1

Molecular formula:

C5H8O3

IUPAC Name:

2-hydroxyethyl acrylate

Test material form:

not specified

Details on test material:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2-Hydroxyethyl acrylate
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): approx. 10 % free acrylic acid

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean body weight at study initiation: 22.9 g (males), 22.8 g (females)


ENVIRONMENTAL CONDITIONS
no details given

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

1600, 800, 640, 500, 400, and 200 µL/kg bw (corresponding to approx. 1618, 809, 647, 506, 404, and 202 mg/kg bw)
Recalculation based on density = 1.011 g/mL

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing:
Cageside observations were performed several times on the day of administration and daily on workdays during observation period. Body weights were determined at test start and termination.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross-pathological investigation

Statistics:

On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 455 mg/kg bw

Remarks on result:

other: density = 1.011 g/mL

Mortality:

At the highest dose, all animals died within 24 hrs after administration. At 809, 647 and 506 mg/kg bw 8/10, 8/10 and 6/10 animals died within 7 days after exposure, respectively. At the two lower doses (404, and 202 mg/kg bw), no mortality was observed.

Clinical signs:

Dyspnoea, saltatory spasms, morphine tail, watery salivation, slight apathy, atonia, half-closed eyes, abdominal position, tremor, twitching, spastic gait, lateral position.

Body weight:

Stagnation of body weight development was observed during the 7-days post-exposure period.

Gross pathology:

Reddened peritoneum, slight intraabdominal adhesions, no substance-related abnormalities.

Any other information on results incl. tables

Mortality:

 

Dose [mg/kg bw]	Conc. [%]	No. of animals/sex	Dead animals / treated animals after
			1 h	24 h	48 h	7 d
1618	16	5 m	0/5	5/5	5/5	5/5
		5 f	0/5	5/5	5/5	5/5
809	8	5 m	0/5	4/5	4/5	5/5
		5 f	0/5	3/5	3/5	3/5
647	8	5 m	0/5	4/5	5/5	5/5
		5 f	0/5	3/5	3/5	3/5
506	8	5 m	0/5	4/5	4/5	4/5
		5 f	0/5	2/5	2/5	2/5
404	4	5 m	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5
202	2	5 m	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5

m: male

f: female