Hydrocarbons, C10-C13, aromatics, <1% naphthalene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 12, 2001 to Mar 16, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The reliability rating is a 1 because the study followed a standard guideline, which describes a procedure designed to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.

Justification for type of information:

The justification for read across is provided as an attachment in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

Deviations:

yes

Remarks:

Minor deviations that were not believed to have affected the outcome of the study.

Principles of method if other than guideline:

Due to the complex nature and limited water solubility of the test substance, the following exceptions to the guideline apply for this study: The concentration of the test substance in solution was not determined prior to use. It was deemed more appropriate to prepare individual treatment solutions by adding the test substance to dilution water and removing the WAF of each mixture for testing than to prepare dilutions of a stock solution. The initial concentration of the test substance was not maintained at 80% in the 2.0 mg/L (78% maintained) and 10 mg/L (76% maintained) treatments throughout the test. Fish were smaller (average 3.3 cm) than the guideline minimum recommended size of 4 cm. Smaller fish were used to help maintain the dissolved oxygen, due to the study design (no headspace). The pH varied by more than one unit during the test, range was 6.3 to 7.8.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples were removed from the fresh solutions at initiation, on Day 0 and 3. Samples were also removed from the old solutions on Day 1, and at termination on Day 4.

Vehicle:

no

Details on test solutions:

Individual treatment solutions were prepared as Water Accommodated Fractions (WAFs). This included adding test substance to 13 L glass aspirator bottles, which contained 13.5 L of laboratory dilution water and mixing for 24 hours with a motorized stirrer at a rate that minimized the vortex so that no emulsion was formed. After mixing, the WAFs were allowed to settle. The test solutions were then siphoned from the bottom of the mixing vessel and used to prepare the individual treatment replicates.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish supplied by Pierce Associates, Inc., West Buxton, ME; age = 10 weeks old; mean wt. = 0.219 g; mean total length = 3.3 cm; test loading = 0.243 g of fish/L.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

Water hardness was 104 mg/L as CaCO3.

Test temperature:

Mean Test temperature was 13.9 Deg C.

pH:

The pH ranged from 6.3 to 7.8 during the study.

Dissolved oxygen:

Dissolved oxygen = 6.2 to 9.1 mg/L

Salinity:

Freshwater

Nominal and measured concentrations:

Nominal treatment levels were: control, 0.43, 0.98, 2.0, 4.8, and 10 mg/L, which measured 0, 0.12, 0.28, 0.51, 0.71, and 0.61 mg/L, respectively, as calculated using the mean of new solution values.

Details on test conditions:

Lighting was 727 to 736 Lux with 16 hrs light and 8 hrs dark.

Two replicates of each treatment were tested in 4 L glass aspirator bottles with 5 fish in each tank. New WAFs for each treatment were prepared as described above for each daily renewal. Renewal of test solution was performed by siphoning out approximately 80% of the old solution and refilling with freshly prepared WAF.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LL50

Effect conc.:

5.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

5.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LL50

Effect conc.:

4.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

3.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No control mortalities or abnormal observations were recorded during the study.

Reported statistics and error estimates:

The 24 to 96-hr LL50 value were determined using a maximum likelihood analysis based on D.J. Finney (1971).

Sublethal observations / clinical signs:

96-hour LL50 = 3.6 mg/L (95% CI Could Not Calculate) based upon nominal values
96-hour LC50 = 0.46 mg/L (95% CI 0.37 to 0.53) based upon mean measured values

Nominal	Measured	Fish Total Mortality
Conc. (mg/L)	Conc. (mg/L)	(@ 24, 48, 72, 96 hrs)**
Control	Control	0, 0, 0, 0
0.43	0.12	0, 0, 0, 0
0.98	0.28	0, 0, 0, 1
2.00	0.51	6, 7, 7, 7
4.80	0.71	9, 9, 10, 10
10.00	0.61	5, 5, 7, 8

*Concentrations based upon mean measured values of the test solutions
**10 fish added to each of the control and treatment levels at test initiation

Validity criteria fulfilled:

yes

Conclusions:

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, <1% naphthalene, produced a 96-hour LL50 value of 3.6 mg/l with rainbow trout (Oncorhynchus mykiss).

Executive summary:

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, <1% naphthalene, produced a 96-hour LL50 value of 3.6 mg/l with rainbow trout (Oncorhynchus mykiss).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13-Jan-1992 to 17-Jan-1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The reliability rating is a 1 because the study followed a standard guideline, which describes a procedure designed to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.

Justification for type of information:

The justification for read across is provided as an attachment in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples were removed from the fresh solutions at initiation, on Day 1, 2, and 3. Samples were also removed from the old solutions on Day 1, and at termination on Day 4.

Vehicle:

no

Details on test solutions:

Individual treatment solutions were prepared as Water Accommodated Fractions (WAFs). This included adding test substance to 20 L glass carboys, which contained 20 L of laboratory dilution water and mixing for 24 hours with a motorized stirrer at a rate that minimized the vortex so that no emulsion was formed. After mixing, the WAFs were allowed to settle. The test solutions were then siphoned from the bottom of the mixing vessel and used to prepare the individual treatment replicates.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish supplied by Aquatic Research Organisms, Hampton, NH; age = 11 weeks old; mean wt. = 0.575 g; mean total length = 3.4 cm; test loading = 0.553 g of fish/L.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

Water hardness was 44 mg/L as CaCO3.

Test temperature:

Test temperature was 12.0 +/- 1.0 Deg C.

pH:

The pH ranged from 7.6 to 7.7 during the study.

Dissolved oxygen:

Dissolved oxygen = 8.9 to 10.0 mg/L for "new" solution and 7.0 to 8.7 mg/L for "old" solution.

Salinity:

Freshwater

Nominal and measured concentrations:

Nominal treatment levels were: control, 0.5, 1.0, 5.0, 10.0, and 50 mg/L, which measured <0.076, 0.481, 0.872, 3.82, 6.11, and 18.2 mg/L, respectively, as calculated using the mean of new solution values.

Details on test conditions:

Lighting was 68 to 70 ft candles with 16 hrs light and 8 hrs dark.

Four replicates of each treatment were tested in 5.2 L glass tanks with 5 fish in each tank. New WAFs for each treatment were prepared as described
above for each daily renewal. Renewal of test solution was performed by siphoning out approximately 86% of the old solution and refilling with
freshly prepared WAF.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI = 1.0 - 5.0

Details on results:

No control mortalities or abnormal observations were recorded during the study.

Reported statistics and error estimates:

The 24-hr LC50 value was determined using the Probit procedure of SAS. The 48-hr LC50 value was determined using the Spearman-Karber Estimation Method. The 72 and 96-hr LC50 values were determined using the Binomial Method.

Sublethal observations / clinical signs:

96-hour LL50 = 3.0 mg/L (95% CI 1.0 to 5.0) based upon nominal values
96-hour LC50 = 2.34 mg/L (95% CI 0.87 to 3.82) based upon measured values of new solutions

Nominal	Measured	Fish Total Mortality
Conc. (mg/L)	Conc. (mg/L)	(@ 24, 48, 72, 96 hrs)**
Control	Control	0, 0, 0, 0
0.5	0.48	0, 0, 0, 0
1.0	0.87	0, 0, 0, 0
5.0	3.82	7, 10, 20, 20
10.0	6.11	19, 20, 20, 20
50.0	18.2	20, 20, 20, 20

*Concentrations based upon measured values of new solutions
**20 fish added to each of the control and treatment levels at test initiation

Validity criteria fulfilled:

yes

Conclusions:

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, >1% naphthalene, produced a 96-hour LL50 of 3.0 mg/l with rainbow trout (Oncorhynchus mykiss).

Executive summary:

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, >1% naphthalene, produced a 96-hour LL50 of 3.0 mg/l with rainbow trout (Oncorhynchus mykiss).

Description of key information

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, <1% naphthalene, produced a 96-hour LL₅₀ value of 3.6 mg/L with rainbow trout (Oncorhynchus mykiss).

Water accommodated fractions of hydrocarbons, C10-C13, aromatics, >1% naphthalene, produced a 96-hour LL₅₀ value of 3.0 mg/L with rainbow trout (Oncorhynchus mykiss).

This data is read across to Hydrocarbons, C11-C15, aromatics, <1% naphthalene.