Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Constituent approach, additivity formula:
48h-EC50 for aquatic invertebrates = 1.02 mg/L for Eucalyptus oil.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.02 mg/L

Additional information

Based on a weight of evidence approach, the acute toxicity of Eucalyptus oil to aquatic invertebrates can be estimated using adequate toxicity data of major constituents, greater than 10% in the mixture. Three constituents were considered, representing 90% of the mixture. To obtain a worst case estimation, the worst case percentages of each constituent were taken into account based on the individual toxicities of these compounds. These data are summarized in the table below:


Worst-case composition

Aquatic invertebrates

48h-EC50, mg/L

Reference in IUCLID 6

Cineol 1,8


> 100

1,8-Cineol, 470-82-6, Short-term toxicity to aquatic invertebrates, Vryenhoef, 2012, RS, W




(d)-limonene, 5989-27-5, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W

Pinene α



alpha-pinene, 7785-26-4, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W

The acute toxicity of Eucalyptus oil to aquatic invertebrates was estimated using the following additivity formula, as recommended in the Regulation (EC) No 1272/2008 (CLP):

∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)


Ci = concentration of component i (weight percentage);

L(E)C50i = LC50 or EC50 for component i, in mg/L;

L(E)C50m = L(E)C50 of the part of the mixture with test data;


Based on data presented above, the 48h-EC50m value for Eucalyptus oil was estimated to be 1.02 mg/L for aquatic invertebrates for a worst case typical mixture. The worst case assumption is validated with the comparable calculations performed on fishes and validated with 2 studies.