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EC number: 203-550-1 | CAS number: 108-10-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
- Reference Type:
- publication
- Title:
- Mutagenicity studies on ketone solvents: methyl ethyl ketone, methyl isobutyl ketone and isophorone
- Author:
- O'donoghue JL, Haworth SR, Curren RD, Kirby PE., Lawlor T et al.
- Year:
- 1 988
- Bibliographic source:
- Mutat. Res., 206, 149-161
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- - missing S. typhimurium strain TA102 or E. coli strain WP2 uvrA, and 2-aminoanthracene was the only compound used to test the efficacy of the S9 fraction for all strains
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-methylpentan-2-one
- EC Number:
- 203-550-1
- EC Name:
- 4-methylpentan-2-one
- Cas Number:
- 108-10-1
- Molecular formula:
- C6H12O
- IUPAC Name:
- 4-methylpentan-2-one
- Details on test material:
- - Name of test material (as cited in study report): Methyl isobutyl Ketone
- Physical state: Clear, non-viscous liquid
- Analytical purity: Not reported
- Lot/batch No.: MA #T1827
- Stability under test conditions: Not reported
- Storage condition of test material: Not reported
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1538, TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-1254 induced rodent liver microsomes
- Test concentrations with justification for top dose:
- Preliminary toxicity assay: 0.015, 0.05, 0.15, 0.5, 1.7, 5.2, 17, 50, 100, or 150 µL/plate (± S9)
Ames assay: 0.04, 0.1, 0.4, 1, or 4 µL/plate (± S9) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: See Table 1
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 37 ºC for 20 minutes
NUMBER OF REPLICATIONS: Triplicate (no independent repeat) - Evaluation criteria:
- Revertant colonies for a given tester strain within a given test article dilution series were counted either entirely by automated colony counter or entirely by hand. Plates with sufficient test article precipitate to interfere with automated colony counting were counted manually. The condition of the background bacterial lawn was evaluated for evidence of test article toxicity by using a dissecting microscope. This toxicity was scored relative to the solvent control plate and recorded along with the revertant count for that plate on the individual strain data forms a code system.
For a test article to be considered positive, it must cause at least a doubling in the mean revertants/plate of at least one tester strain. This increase in the mean number of revertants/plate must be accompanied by a dose response to increasing concentrations of the test article. In those cases where the observed dose-responsive increase in TA1537 or TA1538 revertants/plate is less than three-fold, the response must be reproducible. - Statistics:
- For all replicates plated, an average and standard deviation were calculated. No statistics conducted (revertant colonies counted).
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preliminary toxicity test, doses of 5.2 µL/plate and above, the microcolony lawn was slightly-to-moderately reduced. The background lawn was absent at doses of 17 µL/plate and above.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preliminary toxicity test, doses of 5.2 µL/plate and above, the microcolony lawn was slightly-to-moderately reduced. The background lawn was absent at doses of 17 µL/plate and above.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preliminary toxicity test, doses of 5.2 µL/plate and above, the microcolony lawn was slightly-to-moderately reduced. The background lawn was absent at doses of 17 µL/plate and above.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preliminary toxicity test, doses of 5.2 µL/plate and above, the microcolony lawn was slightly-to-moderately reduced. The background lawn was absent at doses of 17 µL/plate and above.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preliminary toxicity test, doses of 5.2 µL/plate and above, the microcolony lawn was slightly-to-moderately reduced. The background lawn was absent at doses of 17 µL/plate and above.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- negative
- Executive summary:
In a bacterial reverse mutation assay (equivalent to OECD guideline 471), MIBK was tested at doses of 0, 0.04, 0.1, 0.4, 1.0, or 4.0 µL/plate in Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537, and TA 1538 both in the presence and absence of exogenous metabolic activation (Aroclor 1254-induced rat liver S9). The experiment was conducted in triplicate; however, an independent repeat experiment was not performed. Dimethyl sulfoxide (DMSO) was used as the vehicle and positive controls were included in all incubations. No cytotoxicity was observed and noincrease in the reverse mutation rate was observedat any MIBK concentration either in the presence or absence of metabolic activation. Incubation with positive control substances in the presence or absence of metabolic activation resulted in anticipated increases inreverse mutation rates.
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