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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

OECD 211 study on dissociated analogue (acetylacetone) with 21 days NOEC = 18 mg/L readjusted to 42.8 mg/L calcium acetylacetonate based on molecular weight correction.  

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
42.8 mg/L

Additional information

Long-term toxicity in Daphnia magna was assessed during 21 days according to the OECD guidelines 211. The reproduction test was performed in a semi-static system, included 10 vessels per test concentration and 20 vessels for an untreated control group. Each of the vessels contained one neonate (< 24h old) Daphnia magna in 50 mL test medium. The nominal concentrations of Acetyl acetone tested were 1.0, 2.6, 6.8, 18 and 46 mg/L. The study duration was 21 days and the test solutions were renewed three times a week. The daphnids were fed on a daily basis with a Chlorella pyrenoidosa suspension. Every workday the consition of the parental daphnids was recorded, during the reproduction phase the number of living offspring, immobile young and appearance of unhatched (aborted) eggs was recorded. At the end of the test the lengths of the surviving parental daphnids were measured.

During the study samples for Total Organic Carbon (TOC) analyses were taken at the beginning and the end of four intervals of 48 hours. As TOC-analyses generally confirmed correct preparation and showed no significant loss of Carbon in test medium it was decided to base all test results on the nominal concentrations.

Reproduction in the control group at the end of the test period averaged 118 young. The reproduction in the treatment groups up to and including 18 mg/L was comparable to the control group with average offspring numbers between 96 and 130.

Acetyl acetone did not affect survival, reproduction or growth of Daphnia magna at 18 mg/L (readjusted to 42.8 mg/L calcium acetylacetonate based on molecular weight correction) after 21 days of exposure (NOEC).