Carbon tetrachloride

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented study, except for test item description; not under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Embryo-larval toxicity

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Carbon tetrachloride

EC Number:

200-262-8

EC Name:

Carbon tetrachloride

Cas Number:

56-23-5

Molecular formula:

CCl4

IUPAC Name:

tetrachloromethane

Details on test material:

No data

Sampling and analysis

Details on sampling:

No data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: mix by mechanical stirring or homogenization in the mixing chamber

Test organisms

Test organisms (species):

Rana temporaria

Details on test organisms:

TEST ORGANISM
- Common name: European common frog
- Source: Charles Sullivan, Nashville, Tennessee

ACCLIMATION: no data

QUARANTINE (wild caught): no data

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

4 d

Test conditions

Hardness:

95.9 ± 0.9 mg/L CaCO3

Test temperature:

18.6 ± 0.3 °C

pH:

7.7

Dissolved oxygen:

7.2 - 7.4

Nominal and measured concentrations:

0.010 ± 0.004 mg/L
0.076 ± 0.009 mg/L
0.67 ± 0.13 mg/L
10.7 ± 0.5 mg/L
24.0 ± 2.7 mg/L
41.2 ± 4.1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass, devoid of air-water interface, 500 mL,
- Aeration: continuous, supplied by peristaltic pump reservoirs.
- Type of flow-through (e.g. peristaltic or proportional diluter): peristaltic
- Renewal rate of test solution (frequency/flow rate): 200 ml/hr for 500 ml test chamber
- No. of fertilized eggs/embryos per vessel: 50 to 125 eggs/chamber
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): no data



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared by addition of reagent-grade calcium, magnesium, sodium, and potassium salts to distilled, double deionized water
- Alkalinity: ca. 65 mg/L CaCO3
- Ca/mg ratio: 3.8/1
- Conductivity: 176 umhos/cm


VEHICLE CONTROL PERFORMED: not applicable (no vehicle used)


RANGE-FINDING STUDY: no data

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

0 d

Dose descriptor:

LC50

Effect conc.:

4.56 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

other: combined embryo-larval mortality and teratogenesis

Remarks on result:

other: 2.54 - 8.13

Effect concentrations 2

Duration:

4 d

Dose descriptor:

LC50

Effect conc.:

1.16 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

other: combined embryo-larval mortality and teratogenesis

Remarks on result:

other: 0.65 - 1.96

Details on results:

- Mortality/survival at embryo and larval stages: see table 1
- Days to hatch and numbers hatched: see table 1

Results with reference substance (positive control):

Not applicable (no reference substance used)

Any other information on results incl. tables

Table 1: toxicity of CTC to embryo-larval stages

Concentration (mg/L)	% hatchability (1)	% survival normal organisms (2)
		Hatching	4 days posthatching
0.010 ± 0.004	98 (0)	98	94
0.076 ± 0.009	92 (0)	92	82
0.67 ± 0.13	62 (3)	60	52
10.7 ± 0.5	55 (12)	49	37
24.0 ± 2.7	57 (17)	47	19
41.2 ± 4.1	23 (67)	8	0

(1)     Egg hatchability was based on all animals, normal and aberrant, which completed hatching. Frequencies of teratic survivors appearing in hatched populations were expressed parenthetically.

(2)     Normal organisms were defined as those free of gross teratic defects

Applicant's summary and conclusion

Executive summary:

The toxicity of CTC on embryo-larval stages was tested on the amphbian Rana temporaria using a covered flow-througt test system. Exposure started within 30 minutes of fertilization and was mantained through 4 days posthatching. Control eggs were used, too.

Percent of hatchability, as swell as percent survival of normal organims was determined at hatching time (larva stage) and 4 days posthatching (embryo stage).

LC50 was calculated at 0 days and 4 days beyond hatching. The values are 4.56 mg/L and 1.16 mg/L respectively.