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EC number: 500-513-4 | CAS number: 161074-79-9 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- Flash point
- Auto flammability
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
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- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Exposure related observations in humans
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 - 15 Jul 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EU-Guideline 92/69 EWG
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81 SAG
- Source (laboratory, culture collection): originally obtained from Institut für Wasser-, Boden- und Lufthygiene, Berlin. In-house culture
- Age of inoculum (at test initiation): 3 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C +/- 2 °C
- pH:
- Test start: 7.9 - 8.1
Test end: 7.2 - 8.8 - Nominal and measured concentrations:
- nominal: 0.7, 1.5, 3, 6, 12 and 24 mg/L
mean measured: 0.93, 1.8, 3.2, 6.3, 13.8 and 24.5 mg/L (based on a-WAS) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Erlenmeyer flasks
- Aeration: yes
- Initial cells density: 20000 cells/mL
- Control end cells density: 730000 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes according to EU-Guideline 92/69/EWG
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous illumination
- Light intensity and quality: approximately 8000 Lux (white)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer (wave length: 685 nm)
- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: a-WAS concentration
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.93 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: a-WAS concentration
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.95 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Reported statistics and error estimates:
- The EC-values were determined by probit transformation of percental growth inhibition values using regression analysis. Based on these values an additional probit analysis according to Cavalli-Sforza were conducted.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the Category Approach Justification provided in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: a-WAS concentration
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 68891-38-3, 1993c
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
- Remarks:
- Source, key, 68891-38-3, 1993c
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.93 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: a-WAS concentration
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Source, key, 68891-38-3, 1993c
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.95 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material
- Remarks:
- Source, key, 68891-38-3, 1993c
Referenceopen allclose all
Concentration [mg/L] |
Cell density (x 104cells/mL) |
|||
Test duration |
||||
0 h |
24 h |
48 h |
72 h |
|
Control |
2 |
5 |
18 |
73 |
0.93 |
2 |
5 |
19 |
73 |
1.8 |
2 |
5 |
17 |
58 |
3.2 |
2 |
5 |
16 |
59 |
6.3 |
2 |
5 |
16 |
53 |
13.8 |
2 |
4 |
12 |
19 |
24.5 |
2 |
4 |
13 |
14 |
Description of key information
In the key-study an EC50-value for algae (growth rate) of 27.7 mg a.s./L and a NOEC of 0.95 mg a.s./L could be determined after 72 h.
Nevertheless that endpoint is not used for PNEC-calculation as PNEC-values were calculated on the basis of a QSAR-model for chronic toxicity.
Key value for chemical safety assessment
Additional information
The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.
For AES (C12-13, 1-2.5EO) Na (CAS 161074-79-9) no acute test on algae is available. Thus, as a worst-case assumption, read-across is prepared to a study conducted with alcohol C12 -14 ethoxysulfates. In that tests Scenedesmus subspicatus were exposed to different concentrations of alcohol ethoxysulfates C12 -14 (2EO) (CAS 68891 -38 -3; content of active substance in test material: 69.7% a-WAS) in a static system according to the guidelines OECD 201 and EG Guideline 92/69 C.1. The GLP-study resulted in an EC50 (72 h)-value for growth rate of 27 mg/L as a-WAS (corresponding to 27.7 mg a.s./L) (Sasol, 1993c) and is determined as key study.
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