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Acute Fish Toxicity:

Water accommodated fractions of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 96-hour LL50 value of >1000 mg/L and LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in either the control or treatment test systems.

Chronic Fish Toxicity:

The hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value is 0.101 mg/l based on growth.

Acute Invertebrate Toxicity:

The water accommodated fraction (WAF) of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

Water accommodated fractions (WAFs) of hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, did not produce a 50% effect (mortality) with Chaetogammarus marinus at loadings up to 1000 mg/L after a 96-hour exposure. Therefore, the 96-hour LL50 is reported as >1000 mg/L. Mortality was observed in the 3 highest loadings, 100, 320, and 1000 mg/L after 96 hours, but no greater than 40% mortality was demonstrated and the two highest loading levels demonstrated only 20% mortality. Therefore, the 96-hour NOELR for mortality is reported as 32 mg/L. There was also no mortality in the control. These data are used as read-across data to hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics.

Chronic Invertebrate Toxicity:

The hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value is 0.176 mg/l based on reproduction.

Algal Toxicity:

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively.

Microorganisms Toxicity:

The hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated protozoan, Tetrahymena pyriformis, 48-hr EL50 value for this substance is >1000 mg/L based on growth inhibition.