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EC number: 230-391-5 | CAS number: 7085-85-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl cyanoacetate
- EC Number:
- 203-309-0
- EC Name:
- Ethyl cyanoacetate
- Cas Number:
- 105-56-6
- Molecular formula:
- C5H7NO2
- IUPAC Name:
- ethyl cyanoacetate
- Reference substance name:
- Indermil (n-butyl 2-cyanoacrylate, CAS 6606-65-1)
- IUPAC Name:
- Indermil (n-butyl 2-cyanoacrylate, CAS 6606-65-1)
- Reference substance name:
- [CS]000000157834[/CS]
- IUPAC Name:
- [CS]000000157834[/CS]
- Details on test material:
- Batch no. Tank 434
ID no. 3633/81410
Constituent 1
Constituent 2
Constituent 3
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Date of sampling: 10.07.1992 from an domestic sewage plant
- The activated sludge was centrifuged for 10 min, the supernatant was discarded and the sludge was resuspended in a nutrient solution. The sludge was centrifuged and resuspended again. The inoculum was kept aerobic until use. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10.84 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- 2 test vessels with test substance (10.84 mg DOC/l) and inoculum
- Composition of nutrient solution:
8.5 g KH2PO4
21.75 g K2HPO4
33.3 g Na2HPO4 x 2 H2O
20.0 g NH4Cl
22.5 g MgSO4 x 7 H2O
27.5 g CaCl2
0.25 g FeCl3 x 6 H2O
- Test temperature: 22 ± 1°C
- Continuous darkness: yes
- The test vessels were 2000 ml Erlenmeyer flasks loosely closed with aluminum foil
- Sampling frequency: on day 0, 7, 14, 21, 27 and 28
- Sampling method: repeat determination, one acidulated and one outgased sample ( removal of the anorganic carbon)
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 test vessels without any test substance, but with the inoculum
- Control system: 2 test vessels with reference substance (benzoic acid, sodium salt) 9.44 mg DOC/l and inoculum
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference substance benzoic acid, sodium salt achieved within 10 days after a first excess of the 10%-degradation threshold a degradation of 101%. This suggest that the inoculum has an sufficient activity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance Ethyl cyanoacetate achieved in the DOC-DIE AWAY Test within 28 days a DOC degradation of 98 %. Within 10 days after a first excess of the 10 %-degradation threshold the degradation rate is > 70 %. Therefore the test substance Ethyl cyanoacetate can be describe as readily biodegradable.
- Executive summary:
The test substance Ethyl cyanoacetate achieved in the DOC-DIE AWAY Test within 28 days a DOC degradation of 98 %. Within 10 days after a first excess of the 10 %-degradation threshold the degradation rate is > 70 %. Therefore the test substance Ethyl cyanoacetate can be describe as readily biodegradable.
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