Ethyl 2-cyanoacrylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl cyanoacetate

EC Number:

203-309-0

EC Name:

Ethyl cyanoacetate

Cas Number:

105-56-6

Molecular formula:

C5H7NO2

IUPAC Name:

ethyl cyanoacetate

Constituent 2

Reference substance name:

Indermil (n-butyl 2-cyanoacrylate, CAS 6606-65-1)

IUPAC Name:

Indermil (n-butyl 2-cyanoacrylate, CAS 6606-65-1)

Constituent 3

Reference substance name:

[CS]000000157834[/CS]

IUPAC Name:

[CS]000000157834[/CS]

Details on test material:

Batch no. Tank 434
ID no. 3633/81410

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

- Date of sampling: 10.07.1992 from an domestic sewage plant
- The activated sludge was centrifuged for 10 min, the supernatant was discarded and the sludge was resuspended in a nutrient solution. The sludge was centrifuged and resuspended again. The inoculum was kept aerobic until use.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

10.84 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

inorg. C analysis

Details on study design:

TEST CONDITIONS

- 2 test vessels with test substance (10.84 mg DOC/l) and inoculum

- Composition of nutrient solution:
8.5 g KH2PO4
21.75 g K2HPO4
33.3 g Na2HPO4 x 2 H2O
20.0 g NH4Cl
22.5 g MgSO4 x 7 H2O
27.5 g CaCl2
0.25 g FeCl3 x 6 H2O


- Test temperature: 22 ± 1°C
- Continuous darkness: yes
- The test vessels were 2000 ml Erlenmeyer flasks loosely closed with aluminum foil

- Sampling frequency: on day 0, 7, 14, 21, 27 and 28
- Sampling method: repeat determination, one acidulated and one outgased sample ( removal of the anorganic carbon)


CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 test vessels without any test substance, but with the inoculum
- Control system: 2 test vessels with reference substance (benzoic acid, sodium salt) 9.44 mg DOC/l and inoculum

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

98

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance benzoic acid, sodium salt achieved within 10 days after a first excess of the 10%-degradation threshold a degradation of 101%. This suggest that the inoculum has an sufficient activity.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance Ethyl cyanoacetate achieved in the DOC-DIE AWAY Test within 28 days a DOC degradation of 98 %. Within 10 days after a first excess of the 10 %-degradation threshold the degradation rate is > 70 %. Therefore the test substance Ethyl cyanoacetate can be describe as readily biodegradable.

Executive summary:

The test substance Ethyl cyanoacetate achieved in the DOC-DIE AWAY Test within 28 days a DOC degradation of 98 %. Within 10 days after a first excess of the 10 %-degradation threshold the degradation rate is > 70 %. Therefore the test substance Ethyl cyanoacetate can be describe as readily biodegradable.