Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30 %)

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

adult fish: sub(lethal) effects

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

9-Oct-2001 to 30-Oct-2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study was given a reliability rating of 1 because it followed a standard test guideline, which describes a procedure to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

yes

Remarks:

Deviations did not impact the validity of the study.

Principles of method if other than guideline:

Study duration 21 days instead of 14 days as suggested by test guideline.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples were removed from the control and 2500 and 5000 mg/l concentrations on Day 0, 7, 14, and 21 of the study.

Vehicle:

no

Details on test solutions:

Test solutions were prepared as Water Accommodated Fractions (WAF). The test substance was added to test water, and stirred for 23 hours with a magnetic stirrer until equilibrium. The mixing was done at a slow enough speed as to not cause dispersion or emulsification of the undissolved test substance. The solution was allowed to stand for approximately 1 hour, to allow phase separation, before being carefully siphoned out via a glass tube (added prior to the addition of the test substance). Renewal of the test WAF was conducted every 24 hours.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio (Teleostei, Cyprinidae)
Source of supply - MPI Tubingen
Pre-treatment - the animals were held for >12 days, <5% mortality
(light/dark = 14h / 10h; illumination: GroLux fluorescent tubes).
The animals were 3.0 to 3.5 cm in size.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

20.7 to 23.7 dec C

pH:

8.3 to 8.5

Dissolved oxygen:

>75%

Salinity:

freshwater

Nominal and measured concentrations:

Nominal concentrations were: 0, 2500, 5000 mg/l.

Concentration analysis (TOC) of the control and 2500 and 5000 mg/l test concentrations gave results of <10 mg/l. Indicating very low solubility of the test substance.

Details on test conditions:

10 animals/conc.
20 L glass aquaria with aeration via glass pipette.
14h light / 10h dark.
Animals were fed 1 time per day

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

LL50

Effect conc.:

> 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

21 d

Dose descriptor:

NOELR

Effect conc.:

5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Percent Mortality: (at end of test)
Control - 0%
2500 mg/l - 0%
5000 mg/l - 0%

Validity criteria fulfilled:

yes

Conclusions:

The chronic toxicity of Hydrocarbons, C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by toxicity to the zebrafish (Brachydanio rerio) was evaluated in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day LL50 of >5000 mg/l and is not chronically toxic to fish.

Executive summary:

The chronic toxicity of Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by toxicity to the zebrafish (Brachydanio rerio) was evaluated in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day LL50 of >5000 mg/l and is not chronically toxic to fish.

Description of key information

There is no data available for this substance. However, key data is available for the structural analogues Hydrocarbons C11-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) and Hydrocarbons C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%). The data is read across to this substance based on analogue read across and a discussion and report on the read across strategy is provided as an attachment in IUCLID Section 13.

Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects as a Water Accommodated Fraction (WAF) to Brachydanio rerio, with a 21 -day LL50 of >5000 mg/l. It is therefore not chronically toxic to fish.

Hydrocarbons, C16 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects as a Water Accommodated Fraction (WAF) toBrachydanio rerio, with a 21 -day LL50 of >5000 mg/l. It is therefore not chronically toxic to fish.

Additional information

One study report was available and input as an endpoint record. This study is summarised below.

The study from Haltermann (2001a) examined the long-term toxicity of the test substance Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) to Brachydanio rerio in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects to Brachydanio rerio as a Water Accommodated Fraction (WAF) with a 21 -day LL50 of >5000 mg/l. It is therefore not not chronically toxic to fish.

The study from Haltermann (2001b) examined the chronic toxicity of Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by toxicity to the zebrafish (Brachydanio rerio) was evaluated in freshwater. Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) did not show toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day LL50 of >5000 mg/l and is not chronically toxic to fish.