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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods
Qualifier:
according to guideline
Guideline:
other: DIN 38 412 Part 9 (Alga, Growth Inhibition Test)
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Scenedesmus subspicatus CHODAT
Details on test organisms:
-Source (laboratory, culture collection): Algensammlung Göttingen, SAG 86.81
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23+/- 2°C
pH:
pH values varied from 7.98 (lowest concentration) to 3.74 (highest concentration) at the beginning of the experiment, and 8.10 (lowest concentration) to 4.78 (highest concentration) at the end.
Nominal and measured concentrations:
Nominal concentrations: 5, 10, 25, 50, 100, 250 mg/L
Details on test conditions:
replicates for each concentration: 4
Reference substance (positive control):
yes
Remarks:
Kaliumdichromat, Fa. Merck p.a.
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
41.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 15.944-109.945
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
22.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 8.326-61.347
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
35.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 14.604-84.429
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
16.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 6.118-45.0
Duration:
96 h
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
As the neutralised test concentration (250mg/L) showed an inhibition >50% after 72h and 96h it can be concluded that the effects found in this study are likely not due to pH effects.
Growth stimulation and particular fluorescence were not observed.
Results with reference substance (positive control):
not reported
Reported statistics and error estimates:
Statistik method: Tallarida, R.J jacob, LS (1979): The Dose-Response Relation in Pharmacology, S. 98-103, Springer Verlag

pH at LC50: 7 -8

pH values were measured at the beginning and at the end of the test at all treatments

 concentration (mg/L)  pH values     Fluorescenz (F)       cellnumbers/mL *)  (*10 -3)   
   0 h 96h  0h  72h  96h  0h 72h  96h 
 control  8.13  8.30  33  851  2216  12  192  492
 5 7.98   8.10  34 810   2049  12  183  456
 10 7.84   7.96  34 880  2107  12  198  468
 25 7.44   7.78  34 732   1856  12  166  413
 50 5.68   7.72  35 276   339  12  65  79
 100 4.48   7.07  34 48   109  12  15  29
 250 3.74   4.78  34 0   0  12  5  5
 250 (pH control) 7.75   7.86 35  120   127  12  31  33

*)The conversion factor relating fluorescens to cell numbers is:

F(fluorescens)= N(cellnumbers)/mL-4748.470/220.024


Validity criteria fulfilled:
yes
Remarks:
Cell concentration in the control cultures increased by a factor of at  least 16 within a 3-day period
Conclusions:
The toxicity of dicarboxylic acids towards the growth of Scenedesmus subspicatus after a test duration of 72 h according to DIN 38 412, Part 9. was performed and a ErC50 of 41.9 mg/L was determined. 
Executive summary:

The toxicity of dicarboxylic acids towards the growth of Scenedesmus subspicatus after a test duration of 72 h according to DIN 38 412, Part 9. was performed and a ErC50 of 41.9 mg/L was determined. 

Description of key information

The toxicity of carboxylic acids towards the growth of Scenedesmus subspicatus was performed according to DIN 38 412, Part 9. yielding an EC 50 of 41.9 mg/L after 72 hours of exposure (BASF AG, 1989).

Key value for chemical safety assessment

EC50 for freshwater algae:
41.9 mg/L

Additional information

Concerning the toxicity to algae, the fresh water alga Scenedesmus subspicatus was exposed to the mixture of the dicarboxylic acids for 96 hours. The experiment was conducted according to a national standard method DIN 38 412, Part 9. (BASF, 1989) An 72-h EC50 value for effects on growth rate of 41.9 mg/L and an 72-h EC10 of 22.6 mg/L were obtained. The study was characterized by a concentration-dependant decrease of pH. At the end of the 72-hour exposure period, the pH in the control treatment was 8.3, whereas it decreased from 8.1 to 7.7 in the range of concentrations (25.0 - 50.0 mg/L) covering the EC50 value (41.9 mg/L). At concentrations above 100 mg/L the pH shifts to <5 and pH effects cannot be excluded. Furthermore, it should be noticed that the pH value in the control treatment is high compared to the guideline's requirements.