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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
72 h
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted according to guideline method with restriction. Restriction: The stability of the test concentrations was not verified with analytical monitoring.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to guideline
Guideline:
other: DIN 38412/9 (May 1989)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Amides, C8-18 even numbered, N-[3-(dimethylamino)propyl]
EC Number:
930-947-3
Cas Number:
146987-98-6
Molecular formula:
R-CO(NH)(CH2)(CH2)(CH2)N(Me2), whereas R=C8-18 (even numbered)
IUPAC Name:
Amides, C8-18 even numbered, N-[3-(dimethylamino)propyl]

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Source: Pflanzenphysiologisches Institut Göttingen
- Age of inoculum (at test initiation):
- Method of cultivation: Cultivation in Erlenmeyer flasks (300 ml), filled with culture medium. Constant movement on a mechanical shaker. Illumination with white fluorescent tube (2000 lux).

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +- 2 °C
pH:
not reported
Nominal and measured concentrations:
nominal concentrations: control; 0.025; 0.05; 0.075; 0.1; 0.25; 0.5; 0.75 mg/l
Details on test conditions:
TEST SYSTEM
- Initial cells density: 10 000 cells/ ml
- Control end cells density: 271E3 cells/ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: not reported, probably yes


OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: constant illumination
- Light intensity and quality: 2000 lux with white fluorescent tubes


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter at Ex 485; Em 685 nm in 1 ml cuvettes

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.05 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- Exponential growth in the control (for algal test): yes
Reported statistics and error estimates:
statistcal method: linear regression

Any other information on results incl. tables

Avarage cell number [x1000/ml]

Concentration

24 h

48 h

96 h

Control

21

96

271

0.025

23

118

291

0.05

31

77

263

0.075

21

50

230

0.1

13

48

209

0.25

16

40

177

0.5

13

17

43

0.75

11

12

13

Biomass after 72 h:

Concentration

Biomass integral x 1000

% Inhibition

Control

5460

0.025

6276

- 15

0.05

5148

6

0.075

3864

29

0.1

3612

34

0.25

2868

47

0.5

636

88

0.75

108

98

Growth rater after 72 h:

Concentration

Growth rate

% Inhibition

Control

45,8

0.025

46,8

- 2

0.05

45,4

1

0.075

43,5

5

0.1

42,2

8

0.25

39,9

13

0.5

20,3

56

0.75

3,6

92

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Static acute toxicity test according to OECD TG 201 with Scenedesmus subspicatus. Test meets validity criteria of the guideline. No analytical monitoring was made. Therefore the test results are based on nominal concentration.
ErC50 (72 h) = 0.36 mg/l
ErC10 (72 h) = 0.1 mg/l
EbC50 (72 h) = 0.18 mg/l
EbC10 (72 h) = 0.05 mg/l
Executive summary:

Static acute toxicity test according to OECD TG 201 with Scenedesmus subspicatus. Test meets validity criteria of the guideline. No analytical monitoring was made. Therefore the test results are based on nominal concentration.

ErC50 (72 h) = 0.36 mg/l

ErC10 (72 h) = 0.1 mg/l

EbC50 (72 h) = 0.18 mg/l

EbC10 (72 h) = 0.05 mg/l