Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
In this justification, the read-across (bridging) concept is applied, based on the chemical structure of the potential analogues, their toxicokinetic behaviour and other available (eco-)toxicological data. Please refer also to the detailed read-across justification attached in section 13.

The underlying hypothesis for the read-across is that the target and the source substance have similar environmental fate and behaviour and ecotoxicological properties due to their structural similarity, resemblance to their chemical reactivity, and biotransformation products in environmental compartments. In other words, there is a clear chemical analogy (“biotransformation to common compound", scenario 1 of the Read Across Assessment Framework (ECHA 2017)).
The solvation of both methylamine and methylammonium chloride in water results in solutions of the methylammonium cation (“common breakdown product", scenario 1 of the Read Across Assessment Framework (ECHA 2017)). The toxicity of the respective counterion (“non-common compound” – Cl-) is in this case negligible, as Cl does not drive toxicity effects neither in mammalian species nor in aquatic organisms because it is one of the main electrolytes and is required in large amounts in living organisms. So any toxicity of the amounts originated from the methylammonium chloride at maximum dose levels that would be used in ecotoxicity studies is not expected.


source substance:
methylamine hydrochloride (methylammonium chloride)
structural formula: CH5N.ClH
SMILES: [Cl-].C[NH3+]
CAS 593-51-1
purity: not specified

target substance:
structural formula: CH5N
CAS 74-89-5
purity: ≥ 80 – 100 %

No additional information is available on purity of the source and the target substances. Both substances are normally of high purity, containing only minor amounts of impurities that do not influence the read-across validity.

Read across from the structural analogue to the target substance is based on the high structural similarity of the analogue with the substance of interest and the similarity of their environmental fate and behaviour and (eco-) toxicological characteristics.
The target substance MMA (CAS 74-59-5), as well as the source substance MMA-HCl (CAS 593-51-1) belong to the category of the “Aliphatic amines” according to the profiler “US EPA New Chemical Categories” in the OECD QSAR Toolbox v4.1. According to the inclusion rules of this category, the environmental toxicity of amines is related to the length of the hydrophobic carbon chains: the longer (or greater the number of carbons) the chain the more toxic to aquatic organisms (when the number of amines is constant); and the greater the number of amines, the greater the toxicity given a constant carbon chain length (Explanation tool of profiler “US EPA New Chemical Categories” in the OECD QSAR Toolbox v4.1). In this regard, the target and the source substance – bearing identical hydrophobic carbon chains – are expected to have a similar/equal environmental toxicity.
Moreover, the basicity of amines increases with the length of the aliphatic rest due to electron releasing properties of alkyl groups: the higher the pKa value, the weaker the acid, so the stronger the base. Monomethylamine (the primary amine with a central nitrogen atom and 1 methyl group and 2 hydrogen-atoms) is the least basic of the aliphatic (primary) amines. Monomethylammonium chloride (composed also of one methyl group attached to the nitrogen atom, which is regarded as the common structure / functional group) is no longer basic, but neutralised (please also refer to the following paragraphs). Therefore, it has to be kept in mind that MMA-HCL is an example of a worst case read-across according to RAAF, as MMA tested as salt may achieve very high doses because it is not corrosive.
Furthermore, the chemicals are characterized by a common Mode Of Action (MOA) in detail as “narcotic amines” according to Acute aquatic toxicity MOA by OASIS in the OECD QSAR Toolbox v4.1.
Methylamine and methylammonium chloride - as primary aliphatic amines - undergo similar reactions and resemble each other in their physico-chemical properties. The fundamental properties of different amine classes (primary, secondary and tertiary) – basicity and nucleophilicity – are very much the same (Morrison and Boyd, 1987).
Typical reactions of amines are salt formation, alkylation, and conversion into amides and Hofmann elimination from quaternary ammonium salts (Morrison and Boyd, 1987).
As already mentioned above, they are linked by the common functionality of one central nitrogen atom which bears an unshared pair of electrons and tends to share these electrons determining a similar chemical behaviour. This unshared electron pair can accept a proton - forming a substituted ammonium ion. The tendency to share this electron pair underlies the entire chemical behaviour of amines as a group and this was considered as main / basic parameter, which is suitable for read-across within an analogue approach within an/a analogue/category approach.
The dissociation constant of MMA allows the conclusion that virtually all molecules of methylamine - when dissolved in an excess of water - are present as the methylammonium cation. Moreover, the available data of MMA with hydrochloric acid shows clearly that there will be no relevant amounts of the amine available once in contact with the bodies’ fluids. Only the ionic form is the relevant species present. So, in consequence, the solvation of both methylamine and methylammonium chloride in water would result in solutions of the methylammonium cation (“common breakdown product"). Therefore, one must only regard the physico-chemical properties of the respective counterion. Methylamine solutions are accompanied by the hydroxyl anion OH-, resulting in alkaline solutions, whereas the chloride anion of the methylammonium chloride solutions is not expected to trigger significant changes in the pH and exhibit any significant (eco)toxicological effects. Both anions are naturally and ubiquitous occurring ions and are also to a certain extent required for the maintenance of various body functions.
Besides the influence of HCl on the pH value of an aqueous solution, it does not bear a relevant intrinsic property, allowing one in general to focus on the methylammonium cation. Generally, it should be denoted that very commonly in literature there is no differentiation made between MMA and MMA-HCl.
The source substance MMA HCL and the target substance MMA have a certain toxicity potential towards fish, aquatic invertebrates or aquatic algae and cyanobacteria, but both do not have to be classified (no classification and labelling for aquatic acute nor chronic toxicity) according to Regulation (EC) No 1272/2008.
Primary amines are similar in their environmental fate endpoints. The substances are considered to be hydrolytically stable as they contain no chemical groups liable to hydrolysis. Referring to biodegradation, both substances have been shown to be readily biodegradable. The log Koc values are both negative and relatively close and support the read-across approach. Furthermore, the results show that the substances do not have a significant potential for persistence (not P not vP). Primary amines are not expected to evaporate significantly from the water surface to the atmosphere (for MMA HLC < 1 Pa*m /mol). Both substances lack a significant bioaccumulation potential in organisms (not B not vB).
The similar findings (refer also to the data matrix outlined below) for both substances support the conclusion that the identical molecule will be formed from both substances, and this molecule, i.e. the methylammonium cation, is responsible for their behaviour in the environmental compartment and the observed effects. In consequence, the methylammonium cation is what is indeed left to be considered in both cases and similar effects can reasonably be expected when using data from MMA-HCl for the lacking endpoints, compared to the data obtained with MMA.
Hence, MMA-HCl may perfectly serve as a worst case read-across substance for MMA. So, the available data on MMA-HCl can be used to cover all systemic endpoints currently lacking from MMA, making further testing obsolete.

There is data available on the environmental fate and behaviour, ecotoxicological and toxicological properties of MMA. Data on MMA-HCl covers data on Biodegradation, Toxicokinetics, oral Repeated dose toxicity, Genetic toxicity in vitro and in vivo and Toxicity to reproduction/Developmental toxicity. Hence, the identification and discussion of common properties of MMA and MMA-HCl will be mainly based on this and physicochemical data.
The different physical state of the two substances (MMA is - as a pure substance - gaseous at room temperature, MMA-HCl is a solid primary ammonium salt) triggers some differences in the physico-chemical properties like Melting point, Boiling point, Decomposition temperature and Vapour pressure. Nevertheless, regarding the application of both substances, i.e. their distributed form, the gaseous character of MMA becomes less relevant as the substances are usually not applied in their pure forms but rather as aqueous solutions.
The available data for the following physico-chemical properties, which are among others relevant for absorption into living organisms, are very similar. Both substances are small molecules with a low molecular weight of 31.042 (MMA) resp. 67.52 (MMA-HCl), they are both very soluble in water (completely miscible in water (MMA) and at least 1080 mg/L at 20 °C (MMA-HCl)), have a negative logPow (-0.713 (aqueous solution, 25 °C, pH 11.1 - 11.4) (MMA) and -3.82 (MMA-HCl)), and both are readily biodegradable. Although being expected to be hydrolytically stable in the natural environment, they both have a very low potential for bioaccumulation in aquatic and terrestrial organisms. Most importantly, MMA has a pKa of 10.79 at 20 °C (≙ pKb = 3.21), which indicates that methylamine exists almost entirely in the cationic form as methylammonium cation at pH values of 5 to 9.
MMA (not neutralised) has a toxicity potential towards fish and aquatic invertebrates, but does not have to be classified as hazardous. MMA (neutralised) and MMA-HCl have a lower toxicity potential to aquatic organisms and do not have to be considered to be acutely harmful to fish or aquatic invertebrates, nor to microorganisms (no C & L). MMA and MMA-HCL have been shown to be toxic to aquatic algae and cyanobacteria (shown in Desmodesmus subspicatus, Pseudokirchnerella subcapitata, Scenedesmus obliquus and Microcystis aeruginosa). Their aquatic toxicity potential under real environmental conditions has to be judged carefully, since, methylamine and methylamine hydrochloride are readily biodegradable in nature. As such, both can be considered as non-toxic to aquatic organisms and thus do not have to be classified as hazardous as per the CLP classification criteria.
Regarding their toxicity towards mammals, both substances exert their acute toxicity oral toxicity in the same range (Acute tox 4 - H302 for MMA aqueous solution and for MMA HCl) and are also classified as Acute tox 3 - H331 (MMA - gaseous form; MMA - aqueous solution) and Acute tox 4 - H332 (MMA HCl) via the inhalation route of exposure: MMA (gaseous form and aqueous solution) are also classified as STOT SE 3 (C ≥ 5 %). Moreover, MMA is corrosive / irritative to the skin and the eyes (MMA (gas) = Skin Irrit. 2 and Eye Dam 1; MMA (aqueous solution) = Skin Irrit 1B); in the case of MMA –HCl no classification is warranted.
Regarding their repeated dose toxicity, the No-observed-adverse-effect-levels of both substances to differ to a certain extent (NOAEL ≥ 10 versus NOAEL = 500 mg/kg bw/day). Concerning genetic toxicity in vitro, both substances have been shown to be not mutagenic in Gene mutation in bacteria (Ames) tests. Furthermore, MMA and MMA HCl have been tested for their potential to cause gene mutation in mammalian cells. For MMA an older result gives a positive result when tested only without metabolic activation. However, the very recent new investigation conducted with MMA-HCl showed it not to be mutagenic Chinese hamster ovary cells. For MMA HCL there is also data for toxicity to reproduction and developmental toxicity available (NOEL (reproduction/systemic tox) = 500 mg/kg bw/day and NOAEL (maternal/developmental tox.) = 155 mg/kg bw/day).
No data on long-term toxicity to fish; on long-term toxicity to aquatic invertebrates or carcinogenicity were available.
Reason / purpose for cross-reference:
read-across source
other: BOD (NH3)
Sampling time:
2 wk
Details on results:
Indirect Analysis: BOD (NH3) = 84 %
Direct Analysis: TOC = 96 %
HPLC = 100 %
Validity criteria fulfilled:
Interpretation of results:
readily biodegradable
The substance (methylamine hydrochloride) was determined to be readily biodegradable.
Executive summary:

The biodegradability of methylamine hydrochloride was investigated according to MITI-I (OECD 301C) and published by the Official Bulletin of Economy, Trade and Industry (1988). 30 mg/L activated sludge and 100 mg/L of the test substance were used in this test. The time duration was 2 weeks, whereby the indirect analysis showed a result of 84 % degradation, based on BOD (NH3). Direct analysis results are given as 96 %, based on TOC and 100 %, based on HPLC, respectively.

The target substance methylamine and the source substance methylammonium chloride used in this study belong to the group of primary aliphatic amines. The solvation of both, methylamine and methylammonium chloride in water results in solutions of the methylammonium cation (common "breakdown product"). Both respective counterions are naturally and ubiquitous occurring ions and are also to a certain extent required for the maintenance of various body functions. Besides the influence on the pH value of an aqueous solution (OH-), they do not bear a relevant intrinsic property, allowing one in general to focus on the methylammonium cation. The methylammonium cation is believed to act and to be metabolised by the same mechanisms by microorganisms and by other classes of living organisms.

Therefore both substances are expected to follow the same toxicokinetic pattern.

Description of key information

OECD 301C: The substance Monomethylammonium chloride is readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The biodegradability of monomethylamine has been investigated within different experimental settings.

In the key study (Official Bulletin of Economy, Trade and Industry, 1988), the biodegradability of monomethylamine was determined according to MITI-I (OECD 301C). This experiment was conducted with the almost identical substance methylamine hydrochloride. 30 mg/L activated sludge and 100 mg/L of the test substance were used in this test. The time duration was 2 weeks, whereby the indirect analysis showed a result of 84 % degradation, based on BOD (NH3). Direct analysis results are given as 96 %, based on TOC and 100 %, based on HPLC, respectively. The substance was, thus, considered as readily biodegradable.

In a supporting study (Chudoba et al., 1969), the oxygen consumption of activated sludge exposed to methylamine was studied using respirometers (aerobic conditions). The oxygen consumption by endogenous respiration was recorded whereby two different sets of tests were set up (non-adapted sludge; adapted sludge). The exposure period in both sets was 13 days. The test substance concentration amounts to 66.7 mg/L. The removal of methylamine from the test samples by biodegradation was also determined by studying the chemical oxygen demand. Within 13 days the following results are reported for methylamine: BOD/ThOD = 67.8 % (non-adapted sludge); BOD/COD = 93.5 % (adapted sludge).


In another supporting study (BASF AG, 1990), monomethylamine was investigated in a study similar to OECD 301F. The exposure duration was 28 days and the test substance concentration was 400 mg/L, based on test material. Degradation of 55 % occurred within 28 days under aerobic conditions. The authors report that ammonia or ammonium release may lead to an impaired degradation due to a shift in pH. If this shift in pH does not occur, biological degradation is possible. As inoculum domestic, activated sludge (without adaption) was used. According to this test, the substance is not readily biodegradable. However, due to high substance concentration (400 mg/L), it is assumed that the pH of the test solutions has been influenced leading to a decreased degradation rate as mentioned by the authors. pH values are only reported for the end of the test. Neither additional pH values for other points in time nor information about pH adjustments are available.


In another supporting study (Chemservice S.A., 2010), the biodegradability of the substance monomethylamine was calculated based on QSAR methods by using EPIWIN (BIOWIN v4.10). Seven different models are used by this tool to predict an overall result if the substance is readily biodegradable or not. As newest model, Biowin 7 predicts additionally the biodegradation ability under anaerobic conditions. According to the Linear and also Non-linear Model monomethylamine is biodegrading fast. The Ultimate Biodegradation Timeframe is given in weeks, whereas the Primary Biodegradation Timeframe shows days. Both MITI Models predict that the substance is readily biodegradable, which is also the overall conclusion of the calculation. Under anaerobic conditions monomethylamine is suspected to be biodegraded fast as well.