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EC number: 233-788-1 | CAS number: 10361-37-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- March/April 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP-compliant guideline study with the following deviations: - According to the guideline, healthy young adults should be used. The age of the rats was missing in this report. - The analytical purity of the test substance is not indicated in the report.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- (see rational for reliability)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- barium(2+) dichloride dihydrate
- EC Number:
- 600-412-6
- Cas Number:
- 10326-27-9
- Molecular formula:
- BaCl2.2(H2O)
- IUPAC Name:
- barium(2+) dichloride dihydrate
- Test material form:
- solid: crystalline
- Details on test material:
- Test substance: Barium chloride dihydrate
Molecular weight: 208.23 g/mol
Supplier: American Chemical Society
Purity: reagent-grade
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. A. Ivanovas, 7964 Kisslegg/Allgäu
- Age at study initiation: Not reported
- Weight at study initiation: Males: 105-135 g; Females: 105-120 g
- Housing: Group housing of five animals per sex per cage in labelled Makrolon cages (type III) containing sawdust bedding material (Fa. Brandenburg, 2849 Goldenstedt-Arkeburg).
- Diet (ad libitum except overnight prior to and approx. 4 hours after dosing): Pelleted rodent diet (Ssniff R10 pellets, Ssniff Versuchstierdiäten, 4770 Soest/Westf.
- Water (ad libitum): Tap water
- Acclimation period: 5 days before start of treatment
ENVIRONMENTAL CONDITIONS
Animals were housed in a controlled environment.
- Temperature: ca. 20°C
- Relative humidity: 40 - 60 %
- Air changes: Approx. 10 air changes per hour
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 1%
MAXIMUM DOSE VOLUME APPLIED: 5.0 ml/kg
The control animals received the vehicle alone. - Doses:
- 464 mg/kg, 562 mg/kg, 681 mg/kg, 825 mg/kg
- No. of animals per sex per dose:
- 5 male / 5 female
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: On the day of dosing animals were observed for signs of reaction to treatment at frequent intervals. On subsequent days animals were observed at leaste once daily. Individual bodyweights were recorded on the day of dosing and on days 8 and 15.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- R Core Team (2012). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org/
Please refer to the attachment
Results and discussion
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 619 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 506 - 756
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 676 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 590 - 774
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 645 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 612 - 681
- Mortality:
- Number of dead males per dose:
Control: 0/5
464 mg/kg: 0/5
562 mg/kg: 0/5
681 mg/kg: 4/5 (within 6 hours after dosing)
825 mg/kg: 4/5 (within 6 hours after dosing)
Number of dead females per dose:
Control: 0/5
464 mg/kg: 0/5
562 mg/kg: 3/5 (within 6 hours after dosing)
681 mg/kg: 2/5 (within 6 hours after dosing)
825 mg/kg: 5/5 (within 6 hours after dosing) - Clinical signs:
- other: Signs of reaction to treatment included pilo-erection, hunched posture, pallor of extremities, abnormal gait (waddling), increased or decreased respiratory rate, irregular respiration, gasping respiration, increased or decreased motility, diarrhoea, sedat
- Gross pathology:
- Autopsy mainly revealed congestion of liver and spleen. Haemorrhaging and swelling of the glandular gastric mucosa was also observed.
Any other information on results incl. tables
Based on the resuls of this study, an LD50 of 645 mg/kg bw has been calculated for males and females for BaCl2.
According to the test result the substance has to be classified as harmful im swallowed (Xn; R22) due to the fact that mortality was observed at dose levels of > 200 mg/kg bw (according to 67/548/EEC) and toxic category IV (LD50 >300 and<2000 mg/kg bw.) according to GHS.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Based on the results of this study, an LD50 of 645 mg/kg bw has been calculated for males and females for BaCl2.
- Executive summary:
In a GLP compliant acute oral toxicity study, performed according to OECD guideline 401, the test item BaCl2 was administered to groups of 5 male and 5 female Sprague Dawley rats at dose levels of 464, 562, 681 and 825 mg/kg body weight.
Mortalities occurred within 6 hours after dosing. Signs of reaction to treatment included pilo-erection, hunched posture, pallor of extremities, abnormal gait (waddling), increased or decreased respiratory rate, irregular respiration, gasping respiration, increased or decreased motility, diarrhoea, sedation, loss of righting reflex, loss of coordination, paralysis of the hind legs, tremors and coarse body tremors. Complete recovery was evident in surviving animals by day 3 as judged by external appearance and behaviour.
Autopsy mainly revealed congestion of liver and spleen. Haemorrhaging and swelling of the glandular gastric mucosa was also observed.
The animals showed normal weight gain throughout the study.
In conclusion, the LD50 of BaCl2 is calculated to be 645 mg/kg body weight by oral route in the rat.
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