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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-(hydroxymethyl)acrylamide
EC Number:
213-103-2
EC Name:
N-(hydroxymethyl)acrylamide
Cas Number:
924-42-5
Molecular formula:
C4H7NO2
IUPAC Name:
N-(hydroxymethyl)acrylamide
Details on test material:
- Name of test material (as cited in study report): CT 444 90E
- Substance type: organic
- Physical state: liquid
- Analytical purity:
- Impurities (identity and concentrations): 40-44% N-Methylolacrylamide in water (with < 5% acrylamide and < 3% formaldehyde), 0.003% methoxyphenol, 0.0002% copper sulphate
- Composition of test material, percentage of components: 100%
- Purity test date:
- Lot/batch No.: 6EO-841 (Woodbridge)
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
This test was conducted in EPA moderately hard reconstituted water

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
EPA moderately hard water
Test temperature:
15°C
Nominal and measured concentrations:
Nominal concentrations: 0, 625, 1,250, 2,500, 5,000 and 10,000 ppm.
Details on test conditions:
Ten fish (5 fish per replicate)/concentration
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
625 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
625 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
890 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Confidence limits = 625-1250 ppm

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The validity criteria as mentioned in OECD Guideline 203 are met with the exception of the lack of analytical confirmation of the test article concentrations.
Conclusions:
Under the test conditions, the acute LC50 in fish of the test substance at 96 hours was calculated to be 890 ppm and the NOEC is 625 ppm.