Naphthalene-2-sulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study with acceptable restrictions, i.e. no GLP conditions, missing tester strain for crosslinking/ oxidizing agents

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Naphthalenesulfonic acids

EC Number:

268-860-1

EC Name:

Naphthalenesulfonic acids

Cas Number:

68153-01-5

IUPAC Name:

naphthalene-1-sulfonic acid

Constituent 2

Reference substance name:

Naphthalene-2-sulphonic acid

EC Number:

204-375-3

EC Name:

Naphthalene-2-sulphonic acid

Cas Number:

120-18-3

Molecular formula:

C10 H8 O3 S

IUPAC Name:

naphthalene-2-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Naphthalinsulfonsäure
- Analytical purity: ca. 86%
- Lot/batch No.: 46-9714
- Storage condition of test material: Room temperature

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix

Test concentrations with justification for top dose:

0, 20, 100, 500, 2500, 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: [DMSO]
- Justification for choice of solvent/vehicle: the test substance was completely soluble in DMSO.

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with S-9 mix for strains TA 100, TA 98, TA 1537 and TA 1535

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: N-methyl-N'-nitro-N-nitroso-guanidine (MNNG)

Remarks:

without S-9 mix for strains TA 100 and TA 1535

Controls 3

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylendiamine

Remarks:

without S-9 mix for strain TA 98

Controls 4

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 9-aminoacridine chloride monohydrate

Remarks:

without S-9 mix for strain TA 1537

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation

DURATION
- Preincubation period: 20 minutes (only preincubation test)
- Exposure duration: 48 hours at 37 °C in the dark

DETERMINATION OF CYTOTOXICITY
- Method: relative total growth

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results.

Statistics:

no data

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

ADDITIONAL INFORMATION ON CYTOTOXICITY: No bacteriotoxic effect (reduced his- background growth) was observed.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Standard plate test (20 - 5000 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 98	no	22	1.0	no	negative
	yes	33	1.2	no	negative
TA 100	no	100	1.2	no	negative
	yes	121	1.1	no	negative
TA 1535	no	16	0.9	no	negative
	yes	18	1.0	no	negative
TA 1537	no	9	1.1	no	negative
	yes	10	1.1	no	negative
Preincubation test (20 - 5000 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 98	no	20	1.3	no	negative
	yes	34	1.0	no	negative
TA 100	no	110	1.1	no	negative
	yes	117	0.9	no	negative
TA 1535	no	14	1.2	no	negative
	yes	16	1.1	no	negative
TA 1537	no	8	1.3	no	negative
	yes	12	0.9	no	negative