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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Justification for type of information:
Crude sulfate turpentine is a UVCB substance that contains the same main constituents as gum turpentine oil. Therefore, data on crude sulfate turpentine can be extrapolated to gum turpentine oil. See read-across justification document in section 13.
Reason / purpose for cross-reference:
read-across source
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
water-accommodated fraction loading rate
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
8.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
water-accommodated fraction loading rate
Basis for effect:
mobility
Details on results:
- Immobilisation of control: 0

- Other adverse effects control: none

- Abnormal responses: none

- Effect concentrations exceeding solubility of substance in test medium: The tested loading rates exceeded the aqueous solubility of the substance.
Reported statistics and error estimates:
The EL50 (EL = Effective Loading) value was calculated using Probit-analysis (Finney-method, lognormal distribution; confidence limit 95 %, significance level: 0.05). The NOELR was determined by comparison of the responses at each loading rate with the control validity criterion for immobilisation (≤ 10 % immobilisation).

Table 1. Test results

 

Control

2.5

mg/L

5.0

mg/L

10

mg/L

25

mg/L

50

mg/L

 

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Immobilisation 24 h

0/20

0

0/20

0

1/20

5

2/20

10

20/20

100

20/20

100

Immobilisation 48 h

0/20

0

0/20

0

2/20

10

12/20

60

20/20

100

20/20

100

Moults 48 h

16/20

80

13/20

65

11/20

55

6/20

30

0/20

0

0/20

0

Living animals at the surface 48 h

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

Table 2. Results of TOC analysis of test media (mg/L)

Time [h]

Control

2.5 mg/L

10 mg/L

50 mg/L

0

0.4

0.6

0.8

3.3

48

0.2

0.1

1.0

3.1

 

Table 3. Results of GC-MS analysis of test media

Vessel

Time [h]

α-Terpineol
[µg/L]

α-Pinene
[µg/L]

β-Pinene
[µg/L]

3-Carene
[µg/L]

Limonene
[µg/L]

Total

[µg/L]

Control

0

0.214**

< 0.367*

0.227**

< 0.151*

0.212**

< 1.17

Control

48

0.214**

< 0.367*

0.227**

< 0.151*

0.212**

< 1.17

2.5 mg/L

0

2.25

42.9

3.50

9.78

3.45

61.9

2.5 mg/L

48

1.41

33.4

2.82

7.76

2.03

47.4

10 mg/L

0

9.97

432

19.4

80.7

21.6

564

10 mg/L

48

6.04

154

12.7

47.6

13.6

234

50 mg/L

0

57.7

1492

112

299

111

2072

50 mg/L

48

36.7

755

91.2

189

88.2

1161

Remarks:

*   Limit of detection

**   derived from equation of the calibration line (axis intercept); no signal was measured in the controls for α-Terpineol, β-Pinene and Limonene

     Quantitative GC-MS-values are based on results and/or limits of detection

Validity criteria fulfilled:
yes
Conclusions:
A 48h EL50 value of 8.8 mg/L and NOELR of 2.5 mg/L have been determined for the effects of the tested substance on mobility of Daphnia magna.
Executive summary:

An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline 202. Following a preliminary range-finding test, twenty daphnids (four replicates, five daphnids per replicate) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading values of 2.5, 5, 10, 25 and 50 mg/L. The immobility of the daphnids was determined in a semi-static 48-h test by visual observation after 24 and 48 h.

After 24 h of exposure, immobilizations were 0% at 2.5 mg/L (loading), 5% at 5 mg/L (loading), 10% at 10 mg/L (loading) and 100% at 25 and 50 mg/L (loading). After 48 h of exposure, immobilizations were 0% at 2.5 mg/L (loading), 10% at 5 mg/L (loading), 60% at 10 mg/L (loading), 100% at 25 and 50 mg/L (loading).

 

Therefore, the 48h EL50 for the test item to Daphnia magna was estimated to be 8.8 mg/L. The 48h NOELr for the test item to Daphnia magna was estimated to be 2.5 mg/L.

Description of key information

In a test conducted according to OECD Guideline 202 with Crude sulfate turpentine, EL50 and NOELR values of 8.8 and 2.5 mg/L were determined. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
8.8 mg/L

Additional information