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EC number: 200-679-5 | CAS number: 68-12-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: US EPA guideline 660/3-75-009
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Dead fish were counted and removed at 1, 3, 6, 12, and 24 h after initial exposure and at a 24-h period thereafter.
- Vehicle:
- no
- Details on test solutions:
- Water was untreated Lake Superior water used at about 19.8 °C (+/- 2.3 °C). Water quality characteristics were measured using methods described by the American Public Health Association et al. (1975).
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- average weight of test fish: 0.912 ± 0.350 g;
delivered from Lake Mills Fish Hatchery. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Dead fish were counted and removed at 1, 3, 6, 12, and 24 h after initial exposure and at a 24-h period thereafter.
- Hardness:
- hardness: 40.4 - 56.3 mg/L as CaCO3
- Test temperature:
- about 19.8 °C (+/- 2.3 °C)
- pH:
- pH: 7.04 - 7.97
- Dissolved oxygen:
- dissolved oxygen: 54.3 - 88.9 %
- Nominal and measured concentrations:
- Exposure samples were analyzed on a DB-UV spectrophotometer at a wavelenght of 200 nm.
Several test concentrations (up to 5, where appropriate) and a control were tested. - Details on test conditions:
- Groups of 10 fish were tested in glass exposure tanks (20x35x9 cm; containing 6.3L).
Fish were not fed 24 h before or during a test. Dead fish were counted and removed at 1, 3, 6, 12, and 24 h after initial exposure and at a 24-h period thereafter. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 6700-7500 mg/L
- Sublethal observations / clinical signs:
No controls died. DMF-exposed Lepomis macrochirus were stressed and showed mortality within 3 h after initial exposure, deaths continued throughout the test with all fish dead in the highest concentration at 96 h (no values given). The 96 h LC50 and its 95% confidence limits were 7100 (6700 - 7500) mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 (96 h) = 7100 mg/L (95 % CL: 6700-7500 mg/L).
- Executive summary:
The acute toxicity of DMF towards fish (Lepomis macrochirus) was investigated according to US EPA guideline 660/3-75-009.
LC50 (96 h) = 7100 mg/L (95 % CL: 6700-7500 mg/L).
Reference
Description of key information
LC50 (96 h) = 7100 mg/L (95 % CL: 6700-7500 mg/L) for Lepomis macrochirus (US EPA Guideline 660/3-75-009, flow-through, GLP compliance not specified).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 7 100 mg/L
Additional information
Two valid experimental results are available regarding acute toxicity to fish.
In an experiment following US-EPA guideline 660/3 -75 -009, Lepromis macrochirus was used as test organism (Poirier et.al., 1986). The key value for chemical safety assessment is taken out of this study report (LC50 (96h) = 7100 mg/L).
For Leuciscus idus, a LC50 value greater than 10000 mg/L is reported (BASF AG, 1976). In this case the exposure time was 48 hours.
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