Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study without analytical concentration verification
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to same study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
Analytical monitoring:
Test organisms (species):
Daphnia magna
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
Reference substance (positive control):
48 h
Dose descriptor:
Effect conc.:
13 100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Validity criteria fulfilled:
EC50 = 13100 mg/L
Executive summary:

Acute toxicity of DMF to Daphnia magna was examined in a static test according to OECD Guideline 202.

Results: EC50 = 13100 mg/L

Description of key information

EC50 (48 h) = 13100 mg/L for Daphnia magna (OECD Guideline 202, static, non-GLP).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
13 100 mg/L

Additional information

The reported acute toxicity of DMF to Daphnia magna ranges from EC50(48h) >100 up to 14500 mg/L. The EC50 > 100 mg/L (BASF AG, 1992) following OECD Guideline 202 represents for formal reasons the lowest valid EC50 value. However, this value is not taken into account for the chemical safety assessment due to the fact that this was only a limit test. A more realistic value is given by a study conducted by Jarosz, T. (2008). This result (EC50 (48h) = 13100 mg/L) will be used in the risk assessment for N,N-dimethylformamide.

Additionally, a Daphnia magna study is conducted in accordance with US-EPA (1975) EPA-660/3-75-009 under static test conditions. Thereby EC50 were derived for an exposure duration of 24h and 48h: EC50 (24 h): 26300 mg/L; EC50 (48 h): 14500 mg/L