Diethyl 4-oxopentane-1,2-dicarboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well conducted GLP compliant study to international test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl 4-oxopentane-1,2-dicarboxylate

EC Number:

413-860-0

EC Name:

Diethyl 4-oxopentane-1,2-dicarboxylate

Cas Number:

1187-74-2

Molecular formula:

C11H18O5

IUPAC Name:

1,4-diethyl 2-(2-oxopropyl)butanedioate

Details on test material:

- Name of test material (as cited in study report): acetonyl succinic acid diethyl ester
- Physical state: Liquid
- Analytical purity: 96.1%
- Lot/batch No.: A548702
- Expiration date of the lot/batch: 1992-05-25
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature, in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 (control), 37.5, 75.0 and 150.0 mg/L
- Sampling method: Quadruplicate samples taken from test vessels at start (0 hours) and end (48 hours) of the test
- Sample storage conditions before analysis: Deep frozen (-20°C)

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of stock solution in test medium. Aliquots of stock solution added to volumes of test medium to achieve required final test concentration
- Controls: Test medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No evidence noted

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: In-house breeding programme
- Age at study initiation (mean and range, SD): < 24 hours
- Feeding during test: No


ACCLIMATION
- Acclimation period: Not applicable

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

pH:

7.7 - 7.9 at test start (0 hours)
8.0 - 8.3 at test end (48 hours)

Dissolved oxygen:

8.0 - 8.2 mg/L at test start (0 hours)
8.1 - 9.4 mg/L at test end (48 hours)

Nominal and measured concentrations:

At test start:
Nominal (mg/L) / Measured (mg/L)
9.375 /
18.75 /
37.5 / 33.36
75.0 / 67.67
150.0 / 143.5
300 /
600 /

At test end:
Nominal (mg/L) / Measured (mg/L)
9.375 /
18.75 /
37.5 / 35.12
75.0 / 73.46
150.0 / 144.5
300 /
600 /

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250mL capacity; Fill volume 200mL
- Aeration: No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water prepared acording to EU test method


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light/8 hpours dark per day
- Light intensity: 500 - 2000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation, assessed at 24 and 48 hours from test start


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

561 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 452 - 914

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

276 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 228 - 325

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None
- Effect concentrations exceeding solubility of substance in test medium: No

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50: 48 hours - 1.28 mg/L (95% confidence limites: 1.06 - 1.507 mg/L)

Reported statistics and error estimates:

EC50 stimated using Logit model (Cox, D.R., Analysis of Binary Data, Methuen & Co. Ltd. (1977))

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of the substance to Daphnia is 276 mg/L

Executive summary:

The 48 hour EC50 of the substance to Daphnia is 276 mg/L