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EC number: 203-492-7 | CAS number: 107-46-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-10-14 to 1991-10-24
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Guideline study; GLP. Most test concentrations tested were above the limit of water solubility for this compound. Measured concentrations were significantly lower than nominal concentrations, reflecting the low water solubility of the compound.
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1400 (Fish Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 79/831
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling: Analytical determination of concentration was performed at the beginning of the test in the stock solutions and the test media at the beginning and end of the test. Water samples were taken from a point approximately midway between the surface, bottom and sides of each aquarium by means of glass pipettes of 20 mL volume carefully dipped into the solution. The samples were filled into volumetric flasks of 20 mL nominal volume. 0.5 mL n-decane containing the toluene used as internal standard for gas chromatography is added. The flask is closed and then shaken for about one minute. The above operations were performed immediately to minimize the losses of the test substance (M2) due to evaporation and reactions at the water interface. From the n-decane phase on top of the water phase, approximately 0.3 mL was taken by a suitable syringe and filled into a 1 mL vial. The vials were stored in a refrigerator until analysis. The procedure for sampling and preparing the stock solution samples were very similar to those used for the test solutions.
water. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Seven stock solutions were prepared using iso-propanol as a co-solvent. These stock solutions were dosed into the water inlet of the aquaria under the test medium level. The stock solutions were continuously agitated during the test by magnetic stirrers and were dosed at intervals of 36 sec into the water flowing into the respective aquarium by HAMILTON dispensers controlled by a computer. The volurne per dosing cycle was 10 μL.
Test water continuously flowed into the aquaria via an inlet tube at a rate of 10 L/h regulated by a flow-meter. The aquaria were made of glass and had a size of 32 x 36 x 38 cm (L x D x H). The test volumes amounted to 40 L. Thus, the test volume was exchanged 6 times per day. In all the aquaria with the exception of the control, the iso-propanol concentration was 0.1 ml/L. The control aquarium contained only test water. Stock solutions were weighed prior to the start of the dosing system and at test termination to determine the total consumption of stock solution per aquarium. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Length at study initiation (length definition, mean, range and SD): 5.4 +/- 0.4 cm
- Weight at study initiation (mean and range, SD): 1.5 +/- 0.4 g
- Culture conditions: Fish were held and tested under a 16/8 light/dark cycle.
- Observations: Fish were monitored daily for mortality and symptoms of intoxication. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 40 - 60 mg/L as CaCO3
- Test temperature:
- 12+/-1°C
- pH:
- 7.4 - 7.6
- Dissolved oxygen:
- 10.8 - 11.7 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0 (Control), 0 (Solvent control; iso-propanol), 0.16, 0.28, 0.50, 0.89, 1.58, 2.81, and 5.00 mg/L.
Analytical results confirm that stock solutions were 80-90% of nominal values and the test substance was stable for the duration of the test. Measured concentrations ranged from 13-56% of nominal concentrations. The lower percentages were at the higher test concentrations, at or above the limit of water solubility (~0.93 mg/L). - Details on test conditions:
- TEST SYSTEM
- Test vessels: The aquaria were made of glass and had a size of 32 x 36 x 38 cm (L x D x H). The test volumes amounted to 40 L. About 4 hours before the start of the test the aquaria were filled with the respective test medium by starting the water flow and the dosing system.
Immediately before the start of the test.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No. Reconstituted water prepared by adding salt stock solutions to demineralized
water (conductivity < 0.2 μmho/cm).
- Intervals of water quality measurement: Dissolved oxygen and pH were also measured daily. Water temperature was monitored and recorded hourly in the control aquaria. - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.11 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.46 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: calculated independently of report author
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL 1.58-5.00
- Details on results:
- - Mortality of control: No mortalities or symptoms of intoxication occurred in the control or solvent control groups.
- Effect concentrations exceeding solubility of substance in test medium: Most test concentrations tested were above the limit of water solubility for this compound. Measured concentrations were significantly lower than nominal concentrations, reflecting the low water solubility of the compound. - Reported statistics and error estimates:
- The LC50 value was calculated by the binomial probability method.
- Sublethal observations / clinical signs:
Table 1. Results of analysis of test media
Nominal concentration (mg/L)
Day 0 measured concentration (mg/L)
Day 4 measured concentration (mg/L)
Mean measured concentration (mg/L)
0 (Control)
-
-
-
0 (Solvent control)
-
-
-
0.16
0.08
0.09
0.09
0.28
0.10
0.12
0.11
0.50
0.07
0.28
0.18
0.89
0.26
0.21
0.24
1.58
0.37
0.37
0.37
2.81
0.32
0.56
0.44
5.00
0.43
0.74
0.59
Table 2. Test results – percentage mortalities throughout the test
Nominal concentration (mg/L)
4 hours
24 hours
48 hours
72 hours
96 hours
0 (Control)
0
0
0
0
0
0 (Solvent control)
0
0
0
0
0
0.16
0
0
0
0
0
0.28
0
0
0
0
0
0.50
0
0
0
0
0
0.89
0
0
0
0
0
1.58
0
0
0
0
0
2.81
0
0
10
20
40
5.00
0
0
10
70
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hr LC50 value of 3.02 mg/L and NOEC of 0.28 mg/L have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal concentrations. A 96-hr LC50 value of 0.46 mg/L and NOEC of 0.11 mg/L have been re-calculated based on mean measured concentrations.
Reference
Description of key information
A 96-hr LC50 value of 3.02 mg/L and NOEC of 0.28 mg/L have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal concentrations. A 96-hr LC50 value of 0.46 mg/L and NOEC of 0.11 mg/L have been re-calculated based on mean measured concentrations. The results of this test have been selected as the lowest definitive value from two reliable tests.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.46 mg/L
Additional information
A 96-hour LC50 value of 3.02 mg/l and NOEC value of 0.28 mg/l have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal concentrations. A 96-hr LC50 value of 0.46 mg/l and NOEC value of 0.11 mg/l have been re-calculated based on mean measured concentrations (Bayer, 1991).
The results of this test have been selected as key because they give the lowest definitive value from two reliable tests.
In the second reliable test with HMDS:
A 48-hour LC50 value of 1.27 mg/l has been determined for the effects of HMDS on mortality of Oryzias latipes based on nominal concentrations (Kurume Research Laboratories, 1995).
Two further studies are available but they are of unassignable reliability:
A 96-hour LC50 value of >13.5 mg/l has been determined for the effects of HMDS on mortality of Cyprinus carpio based on measured concentrations (Huls Infracor, 1999).
A 9-day LC50 value of >0.095 mg/l has been determined for the effects of HMDS on mortality of Oncorhynchus mykiss based on measured concentrations (DCC, 1990).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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