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EC number: 250-954-9 | CAS number: 32210-23-4
Table 6.1.1/1: Raw data
Nominal concentration (mg/L)
This study was performed according to DIN 38412 Part 15 without GLP statement, to assess the acute toxicity of the test substance to fish Leuciscus idus melanotus, under static conditions.
Fish were exposed to 4 nominal test substance concentrations (10, 13, 16 and 20 mg/L) with solvent during 48 hours and the mortality was recorded.
The 48h-LC50 was determined to be 15 mg/L (95% Confidence limit at 13 -16 mg/L).
Some information are missing to validate this study, such as mortality in control. Validity criteria cannot be verified.
Table 6.1.1/2: Analytical monitoring
Stability control (mg/L)
Actual concentration at 0h (mg/L)
Measured concentration at 24h (mg/L)
Measured concentration at 48h (mg/L)
Measured concentration at 72h (mg/L)
Stock solution - Range finding test
Stock solution - test
Table 6.1.1./3: Raw data
This study was performed according to EU Method C.1 with GLP statement, to assess the acute toxicity of the test substance to fish Cyprinus carpio, under semi-static conditions.
A stability test was performed to measure the concentrations of the test substance in aqueous solution during 72 hours. Results show that the test substance is stable during the test duration.
After a range-finding test, fish were exposed to 4 nominal test substance concentrations (6.0, 7.5, 9.5 and 12 mg/L) with a control medium during 96 hours and the mortality were recorded every day.
The 96h-LC50 was determined to be 8.6 mg/L (95% Confidence limit at 7.7 -9.6 mg/L). The 96h-LC0 and 96h-LC100 were 6.7 and 12 mg/L, respectively.
All validity criteria were fulfilled while mortality in the control was 10% after 96 hours.
EU Method C.1, GLP, key study, validity 1:
96h-LC50 = 8.6 mg/L (95% confidence limit = 7.7-9.6 mg/L).
To assess the short-term toxicity of the registered substance to fish, two studies are available. Among this data, one study is assessed as the key study (Scholz, 1997) and the other study as supporting (Scholz, 1996).
According to the key study, performed according to EU Method C.1 with GLP statement, the fish Cyprinus carpio were exposed to a range of substance concentrations (6.0 to 12.0 mg/L) under semi-static conditions, during 96 hours. The 96h-LC50 based on mortality was determined to be 8.6 mg/L (95% confidence limit at 7.7 -9.6 mg/L).
In the supporting study, solvent was used, the test duration was only 48 hours and no analytical measurements were performed. Furthermore, some information were missing and validity criteria cannot be verified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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