Sulphuric acid, compound with graphite

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study was performed according to internationally accepted guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

not applicable

Molecular formula:

not applicable

IUPAC Name:

not applicable

Details on test material:

Expanded Graphite Powder

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

No test substance related inhibition of growth rate and yield was observed up to the highest loading of 100 mg test item/L.

Executive summary:

A study was performed at the Fraunhofer Institute for Molecular Biology and Applied Ecology (IME) to determine the toxicity of the test item Expanded Graphite Powder on the growth of the unicellular fresh water green alga Pseudokirchneriella subcapitata exposed for a test period of 72 hours under static conditions according to OECD 201. The test item was given in sterilised growth medium (100 mg/L loading) to obtain the maximum achievable test item concentration. For the determination of algal growth three replicates for each concentration and eight replicates for controls (test medium only) were exposed to nominal loadings spaced by a factor of

2 (6.25, 12.5, 25.0, 50.0 and 100 mg test item/L). No test substance related inhibition of growth rate and yield was observed up to the highest loading of 100 mg test item/L.

The EC50 was determined to > 100 mg/L and the NOEC value was determined to ≥ 100 mg/L.