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EC number: 253-575-7 | CAS number: 37640-57-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline and GLP-compliant study
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trione, compound with 1,3,5-triazine-2,4,6-triamine (1:1)
- EC Number:
- 253-575-7
- EC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trione, compound with 1,3,5-triazine-2,4,6-triamine (1:1)
- Cas Number:
- 37640-57-6
- Molecular formula:
- C3H6N6.C3H3N3O3
- IUPAC Name:
- 1,3,5-triazinane-2,4,6-trione; 1,3,5-triazine-2,4,6-triamine
- Test material form:
- solid: particulate/powder
- Details on test material:
- Commercial grade
Constituent 1
- Specific details on test material used for the study:
- Batch no. 52334524U0
Homogeneity: given visual
Storage stability: Expiry date: 15 Sep 2020
Melapur MC25
Identifier: CAS 37640-57-6
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Research Models and Services, Germany GmbH,
- Age at study initiation: about 8 – 12 weeks old
- Weight at study initiation: animals of comparable weight (+/- 20% of the mean body weight)
- Fasting period before study: no
- Housing: single houing or up to 5 animals
- Diet: Kliba laboratory diet, ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature: 20–24 °C
- Humidity: 30-70 %
- Air changes: 15 changes per hour
- Photoperiod: 12 hours light / 12 hours dark
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- >= 3.3 - <= 3.5 µm
- Geometric standard deviation (GSD):
- >= 2.1 - <= 2.6
- Remark on MMAD/GSD:
- Two samples were analyzed.
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: For each test group the dusts were produced inside the inhalation system with a dust generator and compressed air and passed into the inhalation system. The concentrations were adjusted by varying the apertural width rotation of the dosing wheel of the dust generator.
- Exposure chamber volume: 34 L
- Method of holding animals in test chamber: restraining tubes
- Source and rate of air: central air conditioning system, 0.8 m³/h
- Method of conditioning air: Central air conditioning system provided cold air of about 15°C. This cold air passed through an activated charcoal filter, adjusted to room temperature of 20 to 24°C and passed through a second particle filter (H13 (HEPA) Camfil Farr, Germany). The so generated conditioned air was used to generate inhalation atmospheres.
- System of generating particulates/aerosols: The test item was stirred in its container before a sample for dust generation was taken. The test item was desagglomerated in a mixer (mixing for 10 seconds) before introduction into the dust generator via the dosing wheel (Gericke/BASF).
- Method of particle size determination: Stack Sampler Mark III (Andersen). Before sampling, impactor stages were assembled with preweighed glass fiber collecting discs, and equipped with a backup particle filter. The impactor was connected to a vacuum pump, and for each test group samples were taken from the breathing zone of the animals. Sampling occurred 30 minutes (or later) after the beginning of the exposure.
- Treatment of exhaust air: The exhaust air was filtered and conducted into the exhaust air of the building.
TEST ATMOSPHERE
- Brief description of analytical method used: gravimetric, filtration equipment with probe, internal diameter: 7 mm
- Samples taken from breathing zone: yes
VEHICLE
In technical trial runs, various modifications of the dust generation procedure were tested to produce the limit concentration of 5 mg/L. To improve the flowability, 1% AEROSIL® R 972 was added to the test item. AEROSIL® is a brand name of various amorphous silicas produced by Evonik Industries, Frankfurt am Main, Germany. AEROSIL® R 972 is a hydrophobic fused silica, which is used to improve free flow and anti-caking characteristics of powders.
TEST ATMOSPHERE:
- Particle size distribution: Based on cascade impactor measurements. The calculation of particle size distribution was carried out by means of mathematical methods for evaluating particle measurements. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 5 mg/L
The actual measured concentration was 5.057 mg/L - No. of animals per sex per dose:
- 5 rats
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Individual body weight was determined once during the acclimatization period, at the start of the exposure period (Day 0) and at least on Days 1, 3 and 7, and weekly thereafter, or before the sacrifice of the animals at the end of the observation period.
- Clinical observations were recorded for each animal before exposure, separately several times during exposure (usually hourly) and after exposure. At least once daily on the preexposure day and during the post exposure observation period.
- Necropsy of survivors performed: yes - Statistics:
- LC50 values were calculated for males, females and both sexes combined using a binomial test.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5.1 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: No mortality was observed.
- Mortality:
- No lethality was observed in male and female animals during the study period of 14 days.
- Clinical signs:
- other: Laboured respiration was noted during the exposure period (Hour 2 to Hour 4) and fur contaminated with test item was noted on Day 0. No abnormalities were detected in the animals during the post exposure observation period from Day 1 onwards.
- Body weight:
- The mean body weights of the animals decreased on the first post exposure observation days but increased thereafter.
- Gross pathology:
- There were no test item-related macroscopic findings at scheduled necropsy.
Any other information on results incl. tables
Analytical measurements
Exposure conditions: Supply air: 1.5 ± 0.0 m³/h, exhaust air: 0.6 ± 0.0 m³/h,
Test substance preparation: 62.8 g/h, temperature: 22.8 ± 0.3 °C, relative humidity: 22.2 ± 1.3 %
Analytical concentration: 5.075 ± 0.622 mg/L (nominal concentration: 52.3 mg/L)
Particle size analysis: MMAD (µm) / GSD: 3.5 / 2.6 (sample 1), 3.3 / 2.1 (sample 2)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Executive summary:
In a GLP-compliant acute inhalation toxicity study according to OECD 403 (single 4 -hour exposure, nose only; limit test), male and female Wistar rats were exposed to the test item melamincyanurat as a dust (key study, BASF 13I0834/09I058). Mortality, clinical signs and body weights were recorded during the 14 day observation period. At necropsy, a macroscopical examination was conducted. The actual measured test item concentration was 5.057 mg/L. Particle size distributions were well within the respirable range, with mass median aerodynamic diameters (MMADs) of 3.5 and 3.3 μm and geometrical standard deviations of 2.6 and 2.1. There was no mortality. Clinical signs of toxicity comprised of laboured respiration noted during exposure (Hour 2 to Hour 4) and substance contaminated fur (Day 0). No clinical signs were observed from study Day 1 onwards. Mean body weights decreased on the first post exposure observation days but increased thereafter. No gross pathological abnormalities were detected. The 4 -hour LC50 value was deemed as > 5.1 mg/L (calculated based on analytical concentraton) under the conditions of this study.
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